Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer

• Material Type: 博士論文
• Title: Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
• Author/Editor: Tobiume, Makoto; Mitsuhashi, Atsushi; Saijo, Atsuro; Ogino, Hirokazu; Afroj, Tania; Ogawa, Hirohisa; Goto, Hisatsugu; Sato, Seidai; Abe, Akane; Haji, Keiko; Ozaki, Ryohiko; Takizawa, Hiromitsu; Nishioka, Yasuhiko
• Author Heading: Tobiume, Makoto; Mitsuhashi, Atsushi; Saijo, Atsuro; Ogino, Hirokazu; Afroj, Tania; Ogawa, Hirohisa; Goto, Hisatsugu; Sato, Seidai; Abe, Akane; Haji, Keiko; Ozaki, Ryohiko; Takizawa, Hiromitsu; Nishioka, Yasuhiko
• Publication, Distribution, etc.: Spandidos Publications
• Publication Date: 2022-09-30
• Publication Date (W3CDTF): 2022-09-30
• Alternative Title: 肺癌における腫瘍浸潤線維細胞の走化因子とその疾患予後に及ぼす影響についての解析; CHEMOTACTIC FACTORS FOR TUMOR-INFILTRATING FIBROCYTES IN LUNG CANCER
• Periodical title: Oncology Letters
• No. or year of volume/issue: 24 5
• Volume: 24
• Issue: 5
• Pages: 417-
• ISSN (Periodical Title): ISSN : 1792-1074; ISSN : 1792-1082
• Degree grantor/type: 徳島大学
• Date Granted: 2023-02-24
• Dissertation Number: 甲第3677号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: C‑X‑C chemokine receptor type 4; C‑X‑C motif chemokine 12; LUAD; NSCLC; fibrocyte
• Target Audience: 一般
• Note (General): Fibrocytes, which are bone marrow‑derived collagen‑producing cells, have been reported to be involved in pathogenesis of pulmonary fibrosis. Our previous study reported that tumor‑infiltrating fibrocytes play a role in tumor progression and drug resistance in lung cancer. The present study therefore examined chemotactic factors for fibrocytes in tissues of non‑small cell lung cancer (NSCLC) and their prognostic significance. Surgically resected tumor tissues were examined for the expression of chemotactic factors, including C‑X‑C motif chemokine 12 (CXCL12), CCL2, platelet‑derived growth factor (PDGF)‑AA and PDGF‑BB, as well as tumor‑infiltrating fibrocytes by immunostaining. The chemotactic ability of fibrocytes in response to each factor was evaluated using a migration assay by counting the migrated cells microscopically, and expression of receptors for chemotactic factors were analyzed by flow cytometry. The expression of CXCL12, but not CCL2, PDGF‑AA, or PDGF‑BB, was associated with the number of tumor‑infiltrating fibrocytes in lung adenocarcinoma (LUAD), but not lung squamous cell carcinoma (LUSQ). In addition, patients with an increased expression of CXCL12 in LUAD but not LUSQ showed a significantly poorer prognosis compared with those with a decreased expression. However, the expression of CCL2, PDGF‑AA and PDGF‑BB was not correlated with the prognosis of patients with NSCLC. The number of fibrocytes was associated with a poor prognosis in LUAD. Fibrocytes derived from the peripheral blood of healthy subjects as well as patients with lung cancer expressed higher levels of CXCR4 compared with CCR2, PDGF and receptor‑α and receptor‑β. Overall, these results suggested that targeting tumor‑infiltrating fibrocytes via the CXCL12/CXCR4 axis may be a useful strategy for controlling the progression of NSCLC, particularly LUAD.
• Access Restrictions: インターネット公開
• Rights (production): This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.( https://creativecommons.org/licenses/by-nc-nd/4.0/ )
• Related Material (DOI): 10.3892/ol.2022.13537
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/118214/20230515161131472788/k3677_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118214/20230515161131710438/k3677_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118214/20230515161131956733/k3677_fulltext.pdf fulltext