Accumulation of α-synuclein in hepatocytes in nonalcoholic steatohepatitis and its usefulness in pathological diagnosis

• Material Type: 博士論文
• Title: Accumulation of α-synuclein in hepatocytes in nonalcoholic steatohepatitis and its usefulness in pathological diagnosis
• Author/Editor: Kakimoto, Takumi; Hosokawa, Masato; Ichimura-Shimizu, Mayuko; Ogawa, Hirohisa; Miyakami, Yuko; Sumida, Satoshi; Tsuneyama, Koichi
• Author Heading: Kakimoto, Takumi; Hosokawa, Masato; Ichimura-Shimizu, Mayuko; Ogawa, Hirohisa; Miyakami, Yuko; Sumida, Satoshi; Tsuneyama, Koichi
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2023-05-08
• Publication Date (W3CDTF): 2023-05-08
• Alternative Title: 非アルコール性脂肪性肝炎における肝細胞へのα-synucleinの蓄積と病理組織学的診断における有用性
• Periodical title: Pathology - Research and Practice
• No. or year of volume/issue: 247
• Volume: 247
• Pages: 154525-
• ISSN (Periodical Title): ISSN : 0344-0338; ISSN : 1618-0631
• Degree grantor/type: 徳島大学
• Date Granted: 2023-10-26
• Dissertation Number: 甲第3760号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Nonalcoholic fatty liver disease; Nonalcoholic steatohepatitis; Ballooning; α-synuclein; Connexin 32; Pathological diagnosis; Immunostaining
• Target Audience: 一般
• Note (General): Backgrounds: Nonalcoholic steatohepatitis (NASH) is characterized by fat deposition, inflammation, and hepatocellular damage. The diagnosis of NASH is confirmed pathologically, and hepatocyte ballooning is an important finding for definite diagnosis. Recently, α-synuclein deposition in multiple organs was reported in Parkinson’s disease. Since it was reported that α synuclein is taken up by hepatocytes via connexin 32, the expression of α-synuclein in the liver in NASH is of interest. The accumulation of α-synuclein in the liver in NASH was investigated. Immunostaining for p62, ubiquitin, and α-synuclein was performed, and the usefulness of immunostaining in pathological diagnosis was examined. Methods: Liver biopsy tissue specimens from 20 patients were evaluated. Several antibodies against α-synuclein, as well as antibodies against connexin 32, p62, and ubiquitin were used for immunohistochemical analyses. Staining results were evaluated by several pathologists with varying experience, and the diagnostic accuracy of ballooning was compared. Results: Polyclonal α-synuclein antibody, not the monoclonal antibody, reacted with eosinophilic aggregates in ballooning cells. Expression of connexin 32 in degenerating cells was also demonstrated. Antibodies against p62 and ubiquitin also reacted with some of the ballooning cells. In the pathologists’ evaluations, the highest interobserver agreement was obtained with hematoxylin and eosin (H&E)-stained slides, followed by slides immunostained for p62 and α-synuclein, and there were cases with different results between H&E staining and immunostaining Conclusion: These results indicate the incorporation of degenerated α-synuclein into ballooning cells, suggesting the involvement of α-synuclein in the pathogenesis of NASH. The combination of immunostaining including polyclonal α-synuclein may contribute to improving the diagnosis of NASH.
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.prp.2023.154525
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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