Involvement of the OTUB1-YAP1 axis in driving malignant behaviors of head and neck squamous cell carcinoma

• Material Type: 博士論文
• Title: Involvement of the OTUB1-YAP1 axis in driving malignant behaviors of head and neck squamous cell carcinoma
• Author/Editor: Jin, Shengjian; Tsunematsu, Takaaki; Horiguchi, Taigo; Mouri, Yasuhiro; Shao, Wenhua; Miyoshi, Keiko; Hagita, Hiroko; Sarubo, Motoharu; Fujiwara, Natsumi; Qi, Guangying; Ishimaru, Naozumi; Kudo, Yasusei
• Author Heading: Jin, Shengjian; Tsunematsu, Takaaki; Horiguchi, Taigo; Mouri, Yasuhiro; Shao, Wenhua; Miyoshi, Keiko; Hagita, Hiroko; Sarubo, Motoharu; Fujiwara, Natsumi; Qi, Guangying; Ishimaru, Naozumi; Kudo, Yasusei
• Publication, Distribution, etc.: John Wiley & Sons
• Publication Date: 2023-11-20
• Publication Date (W3CDTF): 2023-11-20
• Alternative Title: OTUB1-YAP1経路の頭頸部扁平上皮癌の悪性化への関与
• Periodical title: Cancer Medicine
• No. or year of volume/issue: 12 24
• Volume: 12
• Issue: 24
• Pages: 22156-22169
• ISSN (Periodical Title): ISSN : 2045-7634
• Degree grantor/type: 徳島大学
• Date Granted: 2024-03-22
• Dissertation Number: 甲第3826号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Deubiquitinating enzyme (DUB); head and neck squamous cell carcinoma; invasion; OTUB1; YAP
• Target Audience: 一般
• Note (General): Background: Comprehending the molecular mechanisms underlying head and neck squamous cell carcinoma (HNSCC) is vital for the development of effective treatment strategies. Deubiquitinating enzymes (DUBs), which regulate ubiquitin-dependent pathways, are potential targets for cancer therapy because of their structural advantages. Here we aimed to identify a potential target for HNSCC treatment among DUBs. Methods: A screening process was conducted using RNA sequencing data and clinical information from HNSCC patients in the TCGA database. A panel of 88 DUBs was analyzed to identify those associated with poor prognosis. Subsequently, HNSCC cells were modified to overexpress specific DUBs, and their effects on cell proliferation and invasion were evaluated. In vivo experiments were performed to validate the findings. Results: In HNSCC patients, USP10, USP14, OTUB1, and STAMBP among the screened DUBs were associated with a poor prognosis. Among them, OTUB1 showed the most aggressive characteristics in both in vitro and in vivo experiments. Additionally, OTUB1 regulated the stability and nuclear localization of YAP1, a substrate involved in cell proliferation and invasion. Notably, OTUB1 expression exhibited a positive correlation with the HNSCC-YAP score in HNSCC cells. Conclusions: This study highlights the critical role of OTUB1 in HNSCC progression via modulating YAP1. Targeting the OTUB1-YAP1 axis holds promise as a potential therapeutic strategy for HNSCC treatment.
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article under the terms of the Creative Commons Attribution License, (https://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
• Related Material (DOI): 10.1002/cam4.6735
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/118778/20240522130830173315/k3826_abstract.pdf fulltext

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