Involvement of α-klotho in growth hormone (GH) signaling

• Material Type: 博士論文
• Title: Involvement of α-klotho in growth hormone (GH) signaling
• Author/Editor: Koike, Megumi; Sato, Tetsuhiko; Shiozaki, Yuji; Komiya, Aoi; Miura, Mizuki; Higashi, Ayami; Ishikawa, Akane; Takayanagi, Kaori; Uga, Minori; Miyamoto, Ken-ichi; Segawa, Hiroko
• Author Heading: Koike, Megumi; Sato, Tetsuhiko; Shiozaki, Yuji; Komiya, Aoi; Miura, Mizuki; Higashi, Ayami; Ishikawa, Akane; Takayanagi, Kaori; Uga, Minori; Miyamoto, Ken-ichi; Segawa, Hiroko
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2024
• Publication Date (W3CDTF): 2024
• Alternative Title: 成長ホルモンシグナルにおけるα-klothoの関与
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• No. or year of volume/issue: 74 3
• Volume: 74
• Issue: 3
• Pages: 221-229
• ISSN (Periodical Title): ISSN : 0912-0009; ISSN : 1880-5086
• Degree grantor/type: 徳島大学
• Date Granted: 2024-02-22
• Dissertation Number: 甲第3775号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: growth hormone; α-klotho; kidney; CKD; GH-resistant
• Target Audience: 一般
• Note (General): Growth hormone (GH) exerts multiple effects on different organs directly or via its main mediator, insulin-like growth factor1 (IGF1). In this study, we focused on the novel relationship between GH action and the antiaging hormone α-klotho. Immunofluorescent staining of α-klotho was observed in the renal distal tubules and pituitary glands of somatostatin- and GH-positive cells in wild-type (WT) mice. Treatment of 4-week-old WT mice with GH increased IGF1 mRNA expression in the pituitary gland, liver, heart, kidney, and bone but increased α-klotho mRNA expression only in the pituitary gland, kidney, and bone. Increased α-klotho protein levels were observed in the kidney but not in the pituitary gland. No induction of α-klotho RNA expression by GH was observed in juvenile mice with kidney disease, indicating GH resistance. Furthermore, GH and α-klotho supplementation in HEK293 cells transfected with GHR increased Janus kinase 2 mRNA (a GH downstream signal) expression compared to supple­mentation with GH alone. In conclusion, we suggest that 1) the kidney is the main source of secreted α-klotho, which is detected in blood by the downstream action of GH, 2) α-klotho induction by GH is resistant in kidney disease, and 3) α-klotho might be an enhanced regulator of GH signaling.
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
• Related Material (DOI): 10.3164/jcbn.23-127
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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