Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge

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Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge

Material type: 博士論文

Author: 西尾, 謙宏

Publisher: 大阪歯科学会

Publication date: -

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat p...

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Material Type: 博士論文
Title: Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge
Author/Editor: 西尾, 謙宏
Author Heading: 西尾, 謙宏
Publication, Distribution, etc.: 大阪歯科学会
Alternative Title: アテロコラーゲンスポンジを用いたヒト頬脂肪体由来の脱分化脂肪細胞と脂肪幹細胞の軟骨分化
Degree grantor/type: 大阪歯科大学
Date Granted: 2015-03-06
Dissertation Number: 甲第763号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: Dedifferentiated fat cells
Adipose stem cells
Ceiling culture
Buccal fat pad
Chondrocyte differentiation
Cartilage tissue engineering
Target Audience: 一般
Note (General): We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat pad (BFP). We isolated the cells from two patients who underwent oral and maxillofacial surgery. Chondrocyte differentiation was evaluated based on gene and protein expression analysis in addition to histological analysis of DFATs and ASCs seeded in an atelocollagen sponge and cultured for up to 21 days. The gene expression levels of chondrocyte differentiation markers such as aggrecan, collagen type 2, and SOX9 were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested. Additionally, the levels of the embryonic stem cell markers Nanog, SOX2, and OCT4 were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcian blue-positive, indicating production of sulfated glycosaminoglycans, and was aggrecan-positive. The chondrocyte differentiation ability of human DFATs was higher than that of ASCs. Isolation of DFATs from the BFP offers an aesthetic advantage, as the BFP can be obtained from the oral cavity without surface scarring. Therefore, we propose that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering. This study provides evidence that DFATs from the BFP are an ideal cell source for cartilage tissue engineering.
2014年度
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Access Restrictions: インターネット公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
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Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ