Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

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Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

Material type: 博士論文

Author: 海田, 浩治

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Despite the advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentia...

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Material Type: 博士論文
Title: Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro
Author/Editor: 海田, 浩治
Author Heading: 海田, 浩治
Alternative Title: ヒト脱分化脂肪細胞の骨芽細胞分化誘導にむけたin vitroでの緑茶カテキンの応用
Degree grantor/type: 大阪歯科大学
Date Granted: 2016-03-11
Dissertation Number: 甲第774号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: Human Dedifferentiated Fat Cells
Catechin
Osteogenic
Target Audience: 一般
Note (General): Despite the advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), which is the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25–10 μM) in two osteogenic media with or without 100 nM dexamethasone [OM, OM(DEX)] for 12 days. Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (Col1A1) and runt-related transcription factor 2 (RUNX2), and also increased proliferation and mineralization. Compared with OM(DEX) with EGCG, OM with EGCG induced earlier COL1A1 and RUNX2 expression at day 1 and resulted in lower alkaline phosphatase staining at day 6, but higher mineralization at day 12. OM (DEX) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM without DEX might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide evidence for the potential use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy.
2015
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Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
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Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ