Accelerated generation of human induced pluripotent stem cells from human oral mucosa using episomal plasmid vectors and maternal transcription factor Glis1

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Accelerated generation of human induced pluripotent stem cells from human oral mucosa using episomal plasmid vectors and maternal transcription factor Glis1

Material type: 博士論文

Author: 柏木, 隆宏

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Objective: Induced pluripotent stem cells (iPSCs) possess high pluripotency and differentiation potential and may constitute a possible source of auto...

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Material Type: 博士論文
Title: Accelerated generation of human induced pluripotent stem cells from human oral mucosa using episomal plasmid vectors and maternal transcription factor Glis1
Author/Editor: 柏木, 隆宏
Author Heading: 柏木, 隆宏
Alternative Title: エピソーマルプラスミドベクターと母性転写因子Glis1を用いたヒト口腔粘膜からのヒト人工多能性幹細胞樹立の加速化
Degree grantor/type: 大阪歯科大学
Date Granted: 2016-03-11
Dissertation Number: 甲第776号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: iPSC
integration-free plasmid vector
Glis1
human oral mucosal tissue
Target Audience: 一般
Note (General): Objective: Induced pluripotent stem cells (iPSCs) possess high pluripotency and differentiation potential and may constitute a possible source of autologous stem cells for clinical applications. However, the lengthy reprogramming process (up to 1 month) remains one of the most significant challenges facing standard virus-mediated methodology. The transcription factor Gli-like transcription factor Glis1 is highly expressed in unfertilized eggs and one-cell-stage embryos. In this study, iPSCs were generated using a combination of primary human oral mucosa fibroblasts (HOFs) and episomal plasmid vectors expressing transcription factors including Glis1.Materials and Methods: HOFs were established from 3-mm in diameter oral mucosa tissue using the skin trephine of a 23-year-old Asian man. Human iPSCs were generated from the established HOFs using the following episomal plasmid vectors: pCXLE-hOCT3/4-shp53-F that expresses OCT3/4 and shRNA against p53; pCXLE-hSK that expresses SOX2 and KLF4; pCXLE-hUL that expresses L-MYC and LIN28; and pCXLE-hGlis1 that expresses Glis1.Results: Fifty colonies of human embryonic stem (ES)-like cells were observed as early as 20 days following initial episomal plasmid vector transduction. The resulting cell lines shared several characteristics with human ES cells, including morphology, pluripotency-associated gene and protein markers, karyotype analysis, and the ability to differentiate in vivo into all three germ layers.Conclusions: Our method, combining the use of HOFs and episomal plasmid vectors expressing OCT3/4, SOX2, KLF4, L-MYC, shRNA against p53, LIN28, and Glis1, offers a powerful tool for safely and rapidly generating bona fide human iPSCs and facilitates the application of iPSC technology to biomedical research.
2015
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https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=112&item_no=1&attribute_id=20&file_no=2
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https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=112&item_no=1&attribute_id=20&file_no=1
Access Restrictions: インターネット公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ