High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway

• Material Type: 博士論文
• Title: High-Power, Red Light-Emitting Diode Irradiation Enhances Proliferation, Osteogenic Differentiation and Mineralization of Human Periodontal Ligament Stem Cells via ERK Signaling Pathway
• Author/Editor: 山内, 伸浩
• Author Heading: 山内, 伸浩
• Alternative Title: 高出力、赤色 light-emitting diode 照射は、ERK signaling 経路を介してヒト歯根膜幹細胞の増殖、骨分化および石灰化を増大する
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2018-03-09
• Dissertation Number: 甲第816号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: periodontal ligament｜stem cells｜phototherapy｜low-level light therapy｜regeneration｜MAP kinase signaling system
• Target Audience: 一般
• Note (General): Background: Light-emitting diode (LED) is attracting attention as a new light source for phototherapy. However, its effects on periodontal tissue regeneration remain unknown. The aim of this study was to examine the effects of high-power, red LED irradiation on human periodontal ligament stem cells (PDLSCs), which play an important role in periodontal tissue regeneration.Methods: PDLSCs were derived from adult human third molars. The light source was red LED (peak wavelength: 650 nm). Energy densities ranging from 0 to 10 J/cm2 were tested to determine the optimal dose. PDLSC proliferation was measured using two parameters: live cell protease and ATP levels. After the cells were induced to differentiate, Next, the effect of LED irradiation on osteogenic differentiation and mineralization was examined, with a particular focus on the extracellular signal-regulated kinase (ERK)1/2 signaling pathway using an ERK inhibitor (PD98059).Results: LED irradiation at 8 J/cm2 led to a significant increase in PDLSC proliferation and enhanced Runx2 and Osterix mRNA expression, ALP activity, procollagen type 1 C-peptide and osteocalcin production, calcium deposition and Alizarin Red S staining. In addition, LED induced the activation of ERK1/2, and the effects of LED on PDLSC proliferation, differentiation, and mineralization could be suppressed by treatment with PD98059.Conclusion: The results of this study show that 650-nm high-power, red, LED irradiation increases PDLSCs proliferation, and osteogenic differentiation and mineralization, mediated by ERK1/2 activation. These findings suggest that LED may be a useful tool for periodontal tissue regeneration.; 2017年度
• Access Restrictions: インターネット公開
• Rights (production): CC BY-NC-ND
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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