Study on Photodynamic Therapy with 8-MOP for Oral Mucosal Disease

• Material Type: 博士論文
• Title: Study on Photodynamic Therapy with 8-MOP for Oral Mucosal Disease
• Author/Editor: 石川, 敬彬
• Author Heading: 石川, 敬彬
• Alternative Title: 口腔粘膜疾患への 8-MOP 使用によるフォトダイナミックセラピーの検討
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2018-03-09
• Dissertation Number: 甲第825号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Phototoxicity｜8-MOP｜3D culture
• Target Audience: 一般
• Note (General): Psoralen ultraviolet A (PUVA) therapy using the cytotoxicity of ultraviolet radiation combined with 8-methoxypsoralen (8-MOP) is performed in the field of dermatol-ogy. As there are many disorders common to the oral mucosa and skin, treat-ments for refractory disorders of the skim may also be applicable to oral mucosal disorders. We, therefore, performed phototoxicity tests using 8-MOP primarily on cultured cells from oral tissues.The cell lines used were the mouse Balb/3T3, rat osteosarcoma UMR-106, rat squamous cell carcinoma SCC-158, human oral squamous cell carcinoma HSC-2, and human tongue squamous cell carcinoma HSC-3 and HSC-4. 8-MOP was added to the culture medium in 2-dimensional culture and 3-dimensional cul-ture using porcine Type I collagen gel. The cells were irradiated with ultraviolet light at 375 nm using an LED irradiation system. The control group was not irradiated. After 24-hour static culture, the cell survival rate was determined by MTT assay.In 2-dimensional culture, strong phototoxicity tended to be observed in cells de-rived from tumors. In 3-dimensional culture, phototoxicity varied in strength among cell types, and it was milder than in 2-dimensional culture, suggesting insufficient transmission of ultraviolet light to cells through the collagen gel. In cultured cells derived from tumors, the effects of photoirradiation varied among cell lines. As collagen may have inhibited transmission of ultraviolet light, a method to more ef-ficiently deliver ultraviolet light to cells in deep areas of the body is needed.; 2017年度
• Access Restrictions: インターネット公開
• Rights (production): CC BY-NC-ND
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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