Detection of exosomal miRNA in GCF with chronic periodontitis

Material type: 博士論文

Author: 河東, 里奈

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: MicroRNAs suppress the expression of target genes and extensively regulate the expression of genes involved in growth and disease. The purpose of this...

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Material Type: 博士論文
Title: Detection of exosomal miRNA in GCF with chronic periodontitis
Author/Editor: 河東, 里奈
Author Heading: 河東, 里奈
Alternative Title: 慢性歯周炎特有の歯肉溝滲出液中エクソソームにおけるmiRNAの検出
Degree grantor/type: 大阪歯科大学
Date Granted: 2021-03-05
Dissertation Number: 甲第897号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: MicroRNA
Exosome
Periodontal disease
Gingival crevicular fluid
Target Audience: 一般
Note (General): MicroRNAs suppress the expression of target genes and extensively regulate the expression of genes involved in growth and disease. The purpose of this study was to detect exosomes from gingival crevicular fluid (GCF), and to elucidate disease-specific exosomal miRNA expression in GCF from patients with chronic periodontal disease. Comprehensive microRNA expression analysis was performed using exosomal miRNA in GCF to compare the GCF of patients with chronic periodontitis disease with that of healthy participants. The candidate exosomal miRNAs were analyzed using real-time quantitative polymerase chain reaction (qPCR). The presence of exosomes was observed by measuring the particle size, as well as by detecting CD9/CD63 and observing transmission electron microscope images (TEM). Eleven exosomal miRNAs were identified as differentially expressed more than 8 times lower than in healthy participants by global analysis. Of the miRNA, qPCR analysis revealed that the expression of hsa-mir-204-3p was lower in GCF collected from affected and unaffected sites of patients with chronic periodontal disease, compared with those collected from healthy individuals. This suggests that hsa-mir-204-3p might be associated with periodontal pathology.
2020年度
Source: fulltext
https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=284&item_no=1&attribute_id=20&file_no=2
fulltext
https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=284&item_no=1&attribute_id=20&file_no=1
Access Restrictions: インターネット公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ