Effect of low intensity pulsed ultrasound on osteoclast differentiation

• Material Type: 博士論文
• Title: Effect of low intensity pulsed ultrasound on osteoclast differentiation
• Author/Editor: 東, 友莉
• Author Heading: 東, 友莉
• Alternative Title: 低出力超音波パルス(LIPUS)照射が破骨細胞分化に対する影響について
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2021-03-05
• Dissertation Number: 甲第898号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Low-intensity pulsed ultrasound｜osteoclast differentiation｜RAW264 cells｜RANKL｜orthodontic tooth movement
• Target Audience: 一般
• Note (General): Purpose: For many years, people have attempted to identify ways of efficiently moving teeth in orthodontic therapy to reduce harmful effects. In this study, we investigate whether the non-surgical method of low-intensity pulsed ultrasound (LIPUS) irradiation affects osteoclast differentiation in vitro. Materials and Methods: RAW264 cells were stimulated with RANKL and U0126, and LIPUS irradiation was performed using different ultrasonic outputs, irradiation times, and number of irradiations. Assessments used TRAP staining, TRAP quantitative assays, and western blot analysis. Results: LIPUS irradiation significantly increased osteoclast differentiation rates of RAW264 cells. The rates were significantly higher in the three-time irradiation group compared with the single irradiation group. However, significant differences were not observed between the ultrasonic output. TRAP quantitative assay results showed significantly increased osteoclast differentiation rates with increased irradiation time, however, only in the single irradiation group.Conclusion: These results suggest that an increased number of LIPUS irradiations may improve its effectiveness in orthodontic therapy.; 2020年度
• Access Restrictions: インターネット公開
• Rights (production): CC BY-NC-ND
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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