Development of an assay to determine expression levels of ERBB2 and SIRT1 mRNA in saliva

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Development of an assay to determine expression levels of ERBB2 and SIRT1 mRNA in saliva

Material type: 博士論文

Author: 山本, 慎

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Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: The diagnosis of lifestyle-related diseases and cancer at an early stage is important. The use of saliva, rather than blood testing, for diagnosis has...

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Material Type: 博士論文
Title: Development of an assay to determine expression levels of ERBB2 and SIRT1 mRNA in saliva
Author/Editor: 山本, 慎
Author Heading: 山本, 慎
Alternative Title: 唾液を用いたERBB2およびSIRT1 mRNAの発現レベルを決定するアッセイの開発
Degree grantor/type: 大阪歯科大学
Date Granted: 2021-03-05
Dissertation Number: 甲第909号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: ERBB2｜SIRT1｜mRNA saliva ｜blood
Target Audience: 一般
Note (General): The diagnosis of lifestyle-related diseases and cancer at an early stage is important. The use of saliva, rather than blood testing, for diagnosis has many advantages, especially in terms of its non-invasiveness and convenience. we assessed expression levels of ERBB2, a cancer-related gene, and SIRT1, a gene associated with suppressing aging and improving immunity, using human saliva rather than blood samples. Fasting blood samples and unstimulated saliva samples from passive drooling were collected from healthy volunteers in their twenties, and the levels of markers within each sample were assessed via real-time polymerase chain reaction using the comparative threshold cycle (ΔΔCT) method. The nonparametric Mann–Whitney statistical test was applied to determine median differences. We used β-actin as the reference gene in this study. A significant difference and correlation between blood and saliva transcript levels of SIRT1 (r = 0.344) were observed in volunteers. Taken together, these findings indicate that the transcript levels of ERBB2 and SIRT1 measured from saliva have the capacity to be used as diagnostic biomarkers and may minimize the need for performing blood tests.
2020年度
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https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=296&item_no=1&attribute_id=20&file_no=2
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https://osaka-dent.repo.nii.ac.jp/?action=repository_action_common_download&item_id=296&item_no=1&attribute_id=20&file_no=1
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Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
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Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ