Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model

• Material Type: 博士論文
• Title: Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform,CellSaic, in a Rat Congenital Cleft-Jaw Model
• Author/Editor: 呂, 金釗
• Author Heading: 呂, 金釗
• Alternative Title: ラット先天性裂顎モデルにおける新規細胞移植プラットフォーム CellSaic を使用した歯髄および骨髄間葉系幹細胞の骨形成能の比較
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2022-03-04
• Dissertation Number: 甲第922号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: stem cells｜Cellnest™｜CellSaic｜ rDPSCs｜rBMSCs｜regeneration｜osteogenic differentiation｜bone repair｜rat congenital cleft-jaw model
• Target Audience: 一般
• Note (General): Scaffolds stimulate cell proliferation and differentiation and play major roles in providing growth and nutrition factors in the repair of bone defects. We used the recombinant peptide Cellnest™to prepare the three-dimensional stem cell complex, CellSaic, and evaluated whether CellSaic con taining rat dental pulp stem cells (rDPSCs) was better than that containing rat bone marrow stem cells (rBMSCs). rDPSC-CellSaic or rBMSC-CellSaic, cultured with or without osteogenic induction medium, formed the experimental and control groups, respectively. Osteoblast differentiation was evaluated in vitro and transplanted into a rat model with a congenital jaw fracture. Specimens were collected and evaluated by microradiology and histological analysis. In the experimental group, the amount of calcium deposits, expression levels of bone-related genes (RUNX2, ALP, BSP, and COL1),and volume of mineralized tissue, were significantly higher than those in the control group (p < 0.05).Both differentiated and undifferentiated rDPSC-CellSaic and only the differentiated rBMSC-CellSaic could induce the formation of new bone tissue. Overall, rBMSC-CellSaic and rDPSC-CellSaic made with Cellnest™ as a scaffold, provide excellent support for promoting bone regeneration in rat mandibular congenital defects. Additionally, rDPSC-CellSaic seems a better source for craniofacial bone defect repair than rBMSC-CellSaic, suggesting the possibility of using DPSCs in bone tissue regenerative therapy; 2021年度
• Access Restrictions: インターネット公開
• Rights (production): CC BY-NC-ND
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 大阪歯科大学 : 大阪歯科大学学術リポジトリ

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