口腔がん早期診断用マーカーを同定するための新規発がんモデルの確立

Material type: 博士論文

Author: 河原田, 壮史

Publisher: -

Publication date: -

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 新潟大学,博士（歯学）

View All

Notes on use

Note (General)：: Most oral squamous cell carcinomas (OSCCs) arise from a premalignant lesion, oral epithelial dysplasia; however, useful markers for the early detectio...

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Niigata University Academic Repository
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Niigata University Academic Repository

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
Title: 口腔がん早期診断用マーカーを同定するための新規発がんモデルの確立
Author/Editor: 河原田, 壮史
Author Heading: 河原田, 壮史
Alternative Title: Novel cytological model for the identification of early oral cancer diagnostic markers : The carcinoma sequence model
Degree grantor/type: 新潟大学
Date Granted: 2022-03-23
Dissertation Number: 甲第5026号
Degree Type: 博士（歯学）
Text Language Code: eng
Subject Heading: oral squamous cell carcinoma
oral cancer
bromodomain protein 4
c-Myc
p53
cytology
liquid-based cytology
immunocytochemistry
4-nitroquinoline 1-oxide
Target Audience: 一般
Note (General): Most oral squamous cell carcinomas (OSCCs) arise from a premalignant lesion, oral epithelial dysplasia; however, useful markers for the early detection of OSCC are lacking. The present study aimed to establish a novel experimental model to observe changes in the sequential expression patterns of mRNAs and proteins in a rat model of tongue cancer using liquid-based cytology techniques. Cytology specimens were collected at 2, 5, 8, 11, 14, 17 and 21 weeks from rats treated with 4-nitroquinoline 1-oxide to induce tongue cancer. The expression of candidate biomarkers was examined by performing immunocytochemistry and reverse transcription-quantitative PCR. The percentage of positively stained nuclei was calculated as the labeling index (LI). All rats developed OSCC of the tongue at 21 weeks. The mRNA expression levels of bromodomain protein 4 (Brd4), c-Myc and Tp53 were upregulated during the progression from negative for intraepithelial lesion or malignancy to squamous cell carcinoma (SCC). Brd4- and c-Myc-LI increased in low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion and SCC specimens. p53-LI was significantly increased in SCC specimens. This novel experimental model allowed the observation of sequential morphological changes and the expression patterns of mRNAs and proteins during carcinogenesis. Combining immunocytochemistry with cytology-based diagnoses may potentially improve the diagnostic accuracy of OSCC.
Oncology Letters. 2022, 23(3), 76.
新大院博(歯)第512号
Format (IMT): application/pdf
Source: 本文（fulltext）
https://niigata-u.repo.nii.ac.jp/record/2000642/files/r3ndk512.pdf
Access Restrictions: インターネット公開
Related Material (DOI): 10.3892/ol.2022.13196
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 新潟大学 : 新潟大学学術リポジトリ