Left ventricular mechanical and energetic changes in long-term isoproterenol-induced hypertrophied hearts of SERCA2a transgenic rats.

• Material Type: 博士論文
• Title: Left ventricular mechanical and energetic changes in long-term isoproterenol-induced hypertrophied hearts of SERCA2a transgenic rats.
• Author/Editor: Mitsuyama, Shinichi; Takeshita, Daisuke; Obata, Koji; Zhang, Guo-Xing; Takaki, Miyako
• Author Heading: Mitsuyama, Shinichi; Takeshita, Daisuke; Obata, Koji; Zhang, Guo-Xing; Takaki, Miyako
• Publication, Distribution, etc.: Academic Press / Elsevier
• Publication Date: 2013-06
• Publication Date (W3CDTF): 2013-06
• Alternative Title: SERCA2a過剰発現ラットにおけるイソプロテレノール持続投与誘導肥大心の左室力学的エネルギー学的性質の変化
• Periodical title: Journal of molecular and cellular cardiology
• No. or year of volume/issue: 59
• Volume: 59
• Pages: 95-106
• ISSN (Periodical Title): ISSN : 0022-2828
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2014-03-17
• Dissertation Number: 甲第619号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Heart failure; Ca2 + handling; Oxygen consumption; Excitation–contraction coupling
• Target Audience: 一般
• Note (General): type:Thesis; Overexpression of cardiac sarcoplasmic reticulum Ca2 +-ATPase (SERCA2a) has been suggested as a strategic intervention for cardiac failure. However, its benefit in wild-type (WT) rats with normal SERCA2a levels seems to be small. To investigate whether it would be beneficial in a cardiac failure model with down-regulated SERCA2a levels, we made a cardiac hypertrophy model using isoproterenol infusion (1.2 mg kg− 1 day− 1 for 1 or 4 weeks; TG-ISO1w and TG-ISO4w, respectively) in SERCA2a transgenic (TG) rats and compared these rats with littermate WT rats that underwent the same treatments (WT-ISO1w and WT-ISO4w). We analyzed the left ventricular (LV) mechanoenergetics in the excised heart using our original cross-circulation system. The downward shift of curvilinear LV end-systolic pressure–volume relations (ESPVRs) observed in WT-ISO4w rats was abolished in TG-ISO4w rats. The slope and VO2 intercept of the VO2 (myocardial oxygen consumption per beat)–PVA (systolic pressure–volume area: total mechanical energy per beat) linear relation did not differ in any of the groups. The most important finding was a significantly smaller O2 cost of LV contractility in the TG-ISO4w group, which means that less O2 is needed to exert the same LV contractility, compared with the other groups. The increased ratio of SERCA2a/phospholamban returned to the level of the WT-control group only in the TG-ISO4w group. Longer-term up-regulation of mitochondrial transcription factor A for genes of mitochondrial enzymes producing ATP was observed in TG rats. In conclusion, longer-term overexpression of SERCA2a will be beneficial in the present cardiac failure model with down-regulated SERCA2a levels.; 博士（医学）・甲619号・平成26年3月17日; identifier:Journal of molecular and cellular cardiology Vol.59 p.95-106; identifier:00222828; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2704; identifier:Journal of molecular and cellular cardiology, 59: 95-106
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2704/8/01%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2704/2/02%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2704/3/03%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.yjmcc.2013.02.012
• Periodical Title (NCID): AA00702819; https://ci.nii.ac.jp/ncid/AA00702819
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU