Primary cerebral and cerebellar astrocytes display differential sensitivity to extracellular sodium with significant effects on apoptosis.

• Material Type: 博士論文
• Title: Primary cerebral and cerebellar astrocytes display differential sensitivity to extracellular sodium with significant effects on apoptosis.
• Author/Editor: Takeda, Tomohiko; Makinodan, Manabu; Fukami, Shin-ichi; Toritsuka, Michihiro; Ikawa, Daisuke; Yamashita, Yasunori; Kishimoto, Toshifumi
• Author Heading: Takeda, Tomohiko; Makinodan, Manabu; Fukami, Shin-ichi; Toritsuka, Michihiro; Ikawa, Daisuke; Yamashita, Yasunori; Kishimoto, Toshifumi
• Publication, Distribution, etc.: Wiley-Blackwell
• Publication Date: 2014-06
• Publication Date (W3CDTF): 2014-06
• Alternative Title: 大脳由来と小脳由来の初代培養アストロサイトは、アポトーシスに関連する細胞外ナトリウム感受性が異なる
• Periodical title: Cell biochemistry and function
• No. or year of volume/issue: 32 4
• Volume: 32
• Issue: 4
• Pages: 395-400
• ISSN (Periodical Title): ISSN : 0263-6484
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2014-12-03
• Dissertation Number: 乙第1346号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: astrocyte; sodium ion; apoptosis; hyponatraemia; central pontine myelinolysis; schizophrenia
• Target Audience: 一般
• Note (General): type:Thesis; Central pontine myelinolysis is one of the idiopathic or iatrogenic brain dysfunction, and the most common cause is excessively rapid correction of chronic hyponatraemia. While myelin disruption is the main pathology, as the diagnostic name indicates, a previous study has reported that astrocyte death precedes the destruction of the myelin sheath after the rapid correction of chronic low Na+ levels, and interestingly, certain brain regions (cerebral cortex, hippocampus, etc.) are specifically damaged but not cerebellum. Here, using primary astrocyte cultures derived from rat cerebral cortex and cerebellum, we examined how extracellular Na+ alterations affect astrocyte death and whether the response is different between the two populations of astrocytes. Twice the amount of extracellular [Na+] and voltage-gated Na+ channel opening induced substantial apoptosis in both populations of astrocytes, while, in contrast, one half [Na+] prevented apoptosis in cerebellar astrocytes, in which the Na+–Ca2+ exchanger, NCX2, was highly expressed but not in cerebral astrocytes. Strikingly, the rapid correction of chronic one half [Na+] exposure significantly increased apoptosis in cerebellar astrocytes but not in cerebral astrocytes. These results indicate that extracellular [Na+] affects astrocyte apoptosis, and the response to alterations in [Na+] is dependent on the brain region from which the astrocyte is derived.; 博士（医学）・乙第1346号・平成26年12月3日; Copyright © 2014 John Wiley & Sons, Ltd.; identifier:Cell biochemistry and function Vol.32 No.4 p.395-400; identifier:02636484; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2750; identifier:Cell biochemistry and function, 32(4): 395-400
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1002/cbf.3030
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

Table of Contents

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2750/4/01%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2750/2/02%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2750/7/03%e6%9c%ac%e6%96%87.pdf fulltext