Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells.

• Material Type: 博士論文
• Title: Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells.
• Author/Editor: Fujimura, Takanori; Fujimoto, Takashi; Itaya-Hironaka, Asako; Miyaoka, Tomoko; Yoshimoto, Kiyomi; Yamauchi, Akiyo; Sakuramoto-Tsuchida, Sumiyo; Kondo, Saori; Takeda, Maiko; Tsujinaka, Hiroki; Azuma, Masayuki; Tanaka, Yasuhito; Takasawa, Shin
• Author Heading: Fujimura, Takanori; Fujimoto, Takashi; Itaya-Hironaka, Asako; Miyaoka, Tomoko; Yoshimoto, Kiyomi; Yamauchi, Akiyo; Sakuramoto-Tsuchida, Sumiyo; Kondo, Saori; Takeda, Maiko; Tsujinaka, Hiroki; Azuma, Masayuki; Tanaka, Yasuhito; Takasawa, Shin
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2015-07
• Publication Date (W3CDTF): 2015-07
• Alternative Title: シェーグレン症候群の新規自己抗原であるREG 蛋白の唾液導管細胞における発現誘導にはIL-6/STAT 経路が重要である
• Periodical title: Biochemistry and biophysics reports
• No. or year of volume/issue: 2
• Volume: 2
• Pages: 69-74
• ISSN (Periodical Title): ISSN : 2405-5808
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2015-11-27
• Dissertation Number: 甲第641号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Regenerating gene; Interleukin-6; Sjögren׳s syndrome; Janus kinase; Signal transducer and activator of transcription
• Target Audience: 一般
• Note (General): type:Thesis; The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS.; 博士（医学）・甲第641号・平成27年11月27日; Copyright © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).; identifier:Biochemistry and biophysics reports Vol.2 p.69-74(2015 Jul); identifier:24055808; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3102; identifier:Biochemistry and biophysics reports, 2: 69-74
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3102/1/01_%e7%94%b2641%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3102/2/02_%e7%94%b2641%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3102/3/03_%e7%94%b2641%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.bbrep.2015.05.006
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU