Characterizations of the α₁-adrenoceptor subtypes mediating contractions of the human internal anal sphincter.

• Material Type: 博士論文
• Title: Characterizations of the α₁-adrenoceptor subtypes mediating contractions of the human internal anal sphincter.
• Author/Editor: Owaki, Hiroyuki; Sadahiro, Sotaro; Takaki, Miyako; 大脇, 浩幸; 貞廣, 荘太郎; 高木, 都
• Author Heading: Owaki, Hiroyuki; Sadahiro, Sotaro; Takaki, Miyako; 大脇, 浩幸; 貞廣, 荘太郎; 高木, 都
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2015-04
• Publication Date (W3CDTF): 2015-04
• Alternative Title: ヒト内肛門括約筋収縮に寄与するアドレナリンα₁受容体サブタイプの解明
• Periodical title: Journal of pharmacological sciences
• No. or year of volume/issue: 127 4
• Volume: 127
• Issue: 4
• Pages: 424-429
• ISSN (Periodical Title): ISSN : 1347-8613
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2016-03-15
• Dissertation Number: 乙第1372号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Human internal anal sphincter; α₁-adrenoceptor; Fecal incontinence; Age-related response change; Comparison with inferior mesenteric artery; As a predictor of systemic arterial pressure
• Target Audience: 一般
• Note (General): type:Thesis; Human internal anal sphincter (IAS) is contracted by α₁-adrenoceptor stimulation and thus α₁-adrenoceptor agonists may be useful in treating fecal incontinence. This study characterizes the contribution of α₁-adrenoceptor subtypes in contraction of human IAS and to investigate the age-related risk of patients with fecal incontinence. IAS and inferior mesenteric artery (IMA), as a predictor of systemic arterial pressure, were obtained from 11 patients. Both muscle strips were assessed by isometric-contraction experiments using phenylephrine, further in IAS, in the presence of various subtype selective α₁-adrenoceptor antagonists. Immunohistochemistry and gene expression studies were performed in the same samples. The mean pEC₅₀ values with SEM of phenylephrine in IAS (6.30 ± 0.13) were higher than those of IMA (5.60 ± 0.10). Furthermore, the age-related pEC₅₀ change of IAS was observed between age <70 and ≥70 (6.58 ± 0.13 and 6.07 ± 0.16, respectively (P < 0.05)). In IAS, rightward shift of the concentration–response curves of phenylephrine was observed with three α₁-adrenoceptor antagonists. Each pKв value of silodosin, BMY-7378 and prazosin was 9.36 ± 0.53, 7.28 ± 0.20 and 8.89 ± 0.12, respectively. These pKв values and gene expression studies indicated that α₁ᴀ-adrenoceptor subtypes predominantly contributed to human IAS contraction.; 博士（医学）・乙第1372号・平成28年3月15日; © 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).; identifier:Journal of pharmacological sciences Vol.127 No.4 p.424-429(2015.04); identifier:13478613; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3193; identifier:Journal of pharmacological sciences, 127(4): 424-429
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.jphs.2015.02.016
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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