Bone marrow stromal cell sheets may promote axonal regeneration and functional recovery with suppression of glial scar formation after spinal cord transection injury in rats.

• Material Type: 博士論文
• Title: Bone marrow stromal cell sheets may promote axonal regeneration and functional recovery with suppression of glial scar formation after spinal cord transection injury in rats.
• Author/Editor: Okuda, Akinori; Horii-Hayashi, Noriko; Sasagawa, Takayo; Shimizu, Takamasa; Shigematsu, Hideki; Iwata, Eiichiro; Morimoto, Yasuhiko; Masuda, Keisuke; Koizumi, Munehisa; Akahane, Manabu; Nishi, Mayumi; Tanaka, Yasuhito
• Author Heading: Okuda, Akinori; Horii-Hayashi, Noriko; Sasagawa, Takayo; Shimizu, Takamasa; Shigematsu, Hideki; Iwata, Eiichiro; Morimoto, Yasuhiko; Masuda, Keisuke; Koizumi, Munehisa; Akahane, Manabu; Nishi, Mayumi; Tanaka, Yasuhito
• Publication, Distribution, etc.: American Association of Neurological Surgeons
• Publication Date: 2016-11-25
• Publication Date (W3CDTF): 2016-11-25
• Alternative Title: 骨髄間葉系細胞シートはラット脊髄離断損傷後にグリア瘢痕形成を抑制し、軸索再生と後肢運動機能改善を促進する。
• Periodical title: Journal of neurosurgery. Spine
• No. or year of volume/issue: 26 3
• Volume: 26
• Issue: 3
• Pages: 388-395
• ISSN (Periodical Title): ISSN : 1547-5654
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2016-11-24
• Dissertation Number: 甲第656号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: bone marrow stromal cell; cell sheet; axonal regeneration; spinal cord injury; ascorbic acid; glial scar
• Target Audience: 一般
• Note (General): type:Thesis; OBJECTIVE Transplantation of bone marrow stromal cells (BMSCs) is a theoretical potential as a therapeutic strategy in the treatment of spinal cord injury (SCI). Although a scaffold is sometimes used for retaining transplanted cells in damaged tissue, it is also known to induce redundant immunoreactions during the degradation processes. In this study, the authors prepared cell sheets made of BMSCs, which are transplantable without a scaffold, and investigated their effects on axonal regeneration, glial scar formation, and functional recovery in a completely transected SCI model in rats. METHODS BMSC sheets were prepared from the bone marrow of female Fischer 344 rats using ascorbic acid and were cryopreserved until the day of transplantation. A gelatin sponge (GS), as a control, or BMSC sheet was transplanted into a 2-mm-sized defect of the spinal cord at the T-8 level. Axonal regeneration and glial scar formation were assessed 2 and 8 weeks after transplantation by immunohistochemical analyses using anti-Tuj1 and glial fibrillary acidic protein (GFAP) antibodies, respectively. Locomotor function was evaluated using the Basso, Beattie, and Bresnahan scale. RESULTS The BMSC sheets promoted axonal regeneration at 2 weeks after transplantation, but there was no significant difference in the number of Tuj1-positive axons between the sheet- and GS-transplanted groups. At 8 weeks after transplantation, Tuj1-positive axons elongated across the sheet, and their numbers were significantly greater in the sheet group than in the GS group. The areas of GFAP-positive glial scars in the sheet group were significantly reduced compared with those of the GS group at both time points. Finally, hindlimb locomotor function was ameliorated in the sheet group at 4 and 8 weeks after transplantation. CONCLUSIONS The results of the present study indicate that an ascorbic acid-induced BMSC sheet is effective in the treatment of SCI and enables autologous transplantation without requiring a scaffold.; 博士（医学）・甲第656号・平成28年11月24日; © Copyright 2016 American Association of Neurological Surgeons; The definitive version is available at " http://dx.doi.org/10.3171/2016.8.SPINE16250 "; identifier:Journal of neurosurgery. Spine Vol.26 No.3 p.388-395 (2017 Mar); identifier:15475654; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3282; identifier:Journal of neurosurgery. Spine, 26(3): 388-395
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3171/2016.8.SPINE16250
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3282/1/01_%e7%94%b2656%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

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