Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats.

• Material Type: 博士論文
• Title: Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats.
• Author/Editor: Ueyama, Yoshihiro; Yagyuu, Takahiro; Maeda, Masahiko; Imada, Mitsuhiko; Akahane, Manabu; Kawate, Kenji; Tanaka, Yasuhito; Kirita, Tadaaki
• Author Heading: Ueyama, Yoshihiro; Yagyuu, Takahiro; Maeda, Masahiko; Imada, Mitsuhiko; Akahane, Manabu; Kawate, Kenji; Tanaka, Yasuhito; Kirita, Tadaaki
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2016-12
• Publication Date (W3CDTF): 2016-12
• Alternative Title: 骨形成シートによる顎顔面の再生 : ラットにおける実験研究
• Periodical title: Archives of oral biology Vol. p.
• No. or year of volume/issue: 72
• Volume: 72
• Pages: 138-145
• ISSN (Periodical Title): ISSN : 0003-9969
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2017-03-15
• Dissertation Number: 甲第668号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Bone regeneration; Mandibular defect; Bone marrow-derived stromal cells; Cell sheet
• Target Audience: 一般
• Note (General): type:Thesis; OBJECTIVE: Regeneration of maxillofacial bone defects, characterized by relatively small but complicated shapes, poses a significant clinical challenge. Osteogenic matrix cell sheets (OMCSs) have osteogenic ability and good shaping properties and may be ideal graft materials. Here, we assessed whether implantation of OMCSs could be used to repair maxillofacial bone defects. DESIGN: We adopted a rat mandibular symphysis model. The rat mandible is formed by a paired bone and the central portion consisting of fibrous tissue. There is no bone tissue at the site; accordingly, this site was interpreted as a physiological bone gap and was used for evaluation. Rat bone marrow cells were cultured in medium containing dexamethasone and ascorbic acid phosphate to create OMCSs. The OMCSs were implanted into the rat mandibular symphysis without a scaffold. Microcomputed tomography and histological analyses were conducted after 2, 4, and 8 weeks. RESULTS: Two weeks after implantation, microcomputed tomography images and histological sections showed some sparse granular calcification tissue within the bone gap at the mandibular symphysis. At 4 weeks, the calcification tissue spread, and the gap of the mandibles were continued. At 8 weeks, this continuous new bone tissue was matured. The experimental group showed abundant new bone tissue in the group with OMCS implantation, but not in the group with sham implantation. CONCLUSIONS: Our present results indicated that use of OMCSs may be an optimal approach towards achieving maxillofacial regeneration.; 博士（医学）・甲第668号・平成29年3月15日; Copyright © 2016 Elsevier Ltd. All rights reserved.; identifier:Archives of oral biology Vol.72 p.138-145 (2016 Dec); identifier:00039969; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3321; identifier:Archives of oral biology Vol. p., 72: 138-145
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.archoralbio.2016.08.017
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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