Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats.

• Material Type: 博士論文
• Title: Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats.
• Author/Editor: Gotoh, Daisuke; Torimoto, Kazumasa; Tatsumi, Yoshihiro; Hori, Shunta; Yamada, Atsushi; Miyake, Makito; Morizawa, Yosuke; Aoki, Katsuya; Tanaka, Nobumichi; Hirayama, Akihide; Fujimoto, Kiyohide
• Author Heading: Gotoh, Daisuke; Torimoto, Kazumasa; Tatsumi, Yoshihiro; Hori, Shunta; Yamada, Atsushi; Miyake, Makito; Morizawa, Yosuke; Aoki, Katsuya; Tanaka, Nobumichi; Hirayama, Akihide; Fujimoto, Kiyohide
• Publication, Distribution, etc.: Wiley
• Publication Date: 2017-08-07
• Publication Date (W3CDTF): 2017-08-07
• Alternative Title: PDE5阻害薬であるタダラフィルは糖尿病ラットの膀胱血流を改善し、初期段階に低下する下部尿路機能を回復する
• Periodical title: Neurourology and urodynamics
• No. or year of volume/issue: 37 2
• Volume: 37
• Issue: 2
• Pages: 666-672
• ISSN (Periodical Title): ISSN : 0733-2467
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2018-03-15
• Dissertation Number: 甲第677号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: bladder; ischemia; phosphodiesterase 5; rat
• Target Audience: 一般
• Note (General): type:Thesis; AIMS: To investigate the effect of tadalafil on bladder blood flow and lower urinary tract function in a rat model of diabetes. MATERIALS AND METHODS: We studied female Sprague-Dawley rats and induced diabetes in some using a single intraperitoneal injection of streptozotocin. We divided the rats into nondiabetes (ND), diabetes (D), and diabetes with tadalafil (DT) groups. The rats were raised for an additional 7 weeks after diabetes induction. The DT group received oral tadalafil (2 mg/kg/day) for 7 days before the experiments. At 7 weeks after diabetes induction, we performed cystometry, resected the bladders for immunohistochemistry (hypoxia-inducible factor-1α [HIF-1α] and 8-oxo-2'-deoxyguanosine [8-OHdG] staining), and measured bladder blood supply using a laser blood flow meter. RESULTS: The opening pressure, when the urethra opens and urine flow starts, was significantly lower in the DT group than in the D group (24.9 ± 5.9 vs 43.6 ± 12.3 cmH2 O). The inter-contraction interval was significantly longer in the D group than in the ND and DT groups (1566.2 ± 168.7 vs 702.9 ± 165.2 and 787.4 ± 148.8 s). Immunohistochemistry showed positive staining of the urothelial layer for both HIF-1α and 8-OHdG in the D group, but not in the ND or DT groups. Bladder blood flow was significantly lower in the D group than in the ND or DT groups. CONCLUSIONS: Tadalafil improves bladder blood supply and lower urinary tract function in diabetic rats. Tadalafil may be a promising drug that restores lower urinary tract dysfunction in the early phase of diabetes.; 博士（医学）・甲第677号・平成30年3月15日; © 2017 Wiley Periodicals, Inc.; This is the peer reviewed version of the following article : which has been published in final form at http://dx.doi.org/10.1002/nau.23372. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.; identifier:Neurourology and urodynamics Vol.37 No.2 p.666-672 (2018 Feb); identifier:07332467; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3422; identifier:Neurourology and urodynamics, 37(2): 666-672
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1002/nau.23372
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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