Hemoglobin Vesicles prolong the time to circulatory collapse in rats during apnea.

• Material Type: 博士論文
• Title: Hemoglobin Vesicles prolong the time to circulatory collapse in rats during apnea.
• Author/Editor: Naito, Yusuke; Sakai, Hiromi; Inoue, Satoki; Kawaguchi, Masahiko
• Author Heading: Naito, Yusuke; Sakai, Hiromi; Inoue, Satoki; Kawaguchi, Masahiko
• Publication, Distribution, etc.: BioMed Central
• Publication Date: 2017-03-14
• Publication Date (W3CDTF): 2017-03-14
• Alternative Title: ヘモグロビン小胞体は無呼吸ラットにおいて循環虚脱までの時間を延長させる
• Periodical title: BMC anesthesiology
• No. or year of volume/issue: 17 1
• Volume: 17
• Issue: 1
• Pages: Article No.44-
• ISSN (Periodical Title): ISSN : 1471-2253
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2018-03-15
• Dissertation Number: 甲第680号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Hemoglobin Vesicles; Artificial red blood cells; Apnea; Hypoxia
• Target Audience: 一般
• Note (General): type:Thesis; BACKGROUND: Hemoglobin vesicles (HbV) are hemoglobin-based oxygen carriers manufactured by liposome encapsulation of hemoglobin molecules. We hypothesised that the infusion of oxygenated HbV could prolong the time to circulatory collapse during apnea in rats. METHODS: Twenty-four Sprague-Dawley rats were randomly divided into four groups (Air, Oxy, NS and HbV). The rats were anaesthetized with isoflurane and the trachea was intubated using 14-gauge intravenous catheters. Rats in the Air group were mechanically ventilated with 1.5% isoflurane in room air, and those in other groups received 1.5% isoflurane in 100% oxygen. Mechanical ventilation was withdrawn 1 min after the administration of rocuronium bromide to induce apnea. After 30 s, 6 mL saline and HbV boluses were infused at a rate of 0.1 mL/s in the NS and HbV groups, respectively. Circulatory collapse was defined as a pulse pressure < 20 mmHg and the time to reach this point (PP20) was compared between the groups. The results were analysed via a one-way analysis of variance and post-hoc Holm-Sidak test. RESULTS: PP20 times were 30.4 ± 4.2 s, 67.5 ± 9.7 s, 95 ± 17.3 s and 135 ± 38.2 s for the Air (ventilated in room air with no fluid bolus), Oxy (ventilated with 100% oxygen with no fluid bolus), NS (ventilated with 100% oxygen with a normal saline bolus), and HbV (ventilated in 100% oxygen with an HbV bolus) groups, respectively, and differed significantly between the four groups (P = 0.0001). The PP20 times in the HbV group were significantly greater than in the Air (P = 0.0001), Oxy (P = 0.007) and NS (P = 0.04) groups. CONCLUSION: Infusion of oxygenated HbV prolongs the time to circulatory collapse during apnea in rats.; 博士（医学）・甲第680号・平成30年3月15日; © The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.; identifier:BMC anesthesiology Vol.17 No.1 Article No.44 (2017 Mar); identifier:14712253; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3425; identifier:BMC anesthesiology, 17(1): Article No.44
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1186/s12871-017-0338-y
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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