Aquaporin-2 plays an important role in water transportation through the bladder wall in rats.

• Material Type: 博士論文
• Title: Aquaporin-2 plays an important role in water transportation through the bladder wall in rats.
• Author/Editor: Morizawa, Yosuke; Torimoto, Kazumasa; Hori, Shunta; Gotoh, Daisuke; Nakai, Yasushi; Miyake, Makito; Hirayama, Akihide; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Author Heading: Morizawa, Yosuke; Torimoto, Kazumasa; Hori, Shunta; Gotoh, Daisuke; Nakai, Yasushi; Miyake, Makito; Hirayama, Akihide; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Publication, Distribution, etc.: John Wiley and Sons, Inc.
• Publication Date: 2018-11
• Publication Date (W3CDTF): 2018-11
• Alternative Title: ラット膀胱での水吸収におけるアクアポリン-2の役割
• Periodical title: Neurourology and urodynamics
• No. or year of volume/issue: 37 8
• Volume: 37
• Issue: 8
• Pages: 2434-2440
• ISSN (Periodical Title): ISSN : 0733-2467
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2019-03-15
• Dissertation Number: 甲第697号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: aquaporin; bladder; desmopressin; intravesical fluid volume; water transportion
• Target Audience: 一般
• Note (General): type:Thesis; AIM: We investigated the role of the bladder wall in permeating water, focusing on aquaporins. METHODS: Female Sprague-Dawley rats weighing 300 g were used to investigate the role of the bladder wall in saline permeation. Changes in intravesical fluid volume and sodium concentration were measured in the desmopressin acetate hydrate-loaded and control groups 3 h after administration. Bladders were resected to measure aquaporin-1, 2, and 3 gene expression using qRT-PCR. Additionally, the change of aquaporin-2 expression was measured using Western blotting and immunohistochemistry in intravesical aquaporin-2 siRNA-treated and control groups. RESULTS: Although the intravesical fluid volume and sodium concentration significantly decreased from 0 to 3 h (1.00 ± 0.00 vs 0.83 ± 0.08 mL, 157.80 ± 1.30 vs 146.8 ± 1.92 mEq/mL, P < 0.01, respectively in the control group), administration of desmopressin did not affect the extent of volume change. Aquaporin-2 expression was significantly higher in the 3-h distended bladders than in the empty bladder. Aquaporin-2 siRNA treatment suppressed aquaporin-2 expression and the change of intravesical fluid volume from 0 to 3 h (1.00 ± 0.00 and 0.99 ± 0.02 mL), which was related to the suppression of sodium concentration change in comparison with control siRNA treatment (149.6 ± 2.4 vs 143.6 ± 3.67 mEq/mL, P < 0.05). CONCLUSIONS: The rat urinary bladder absorbs water and salts under the full-filled condition. Aquaporin-2 plays an important role in the transport of water, accompanied by sodium concentration change. We demonstrated a part of the bladder absorption mechanism, which may lead to development of a new method for regulating bladder storage function.; 博士（医学）・甲第697号・平成31年3月15日; © 2018 Wiley Periodicals, Inc.; This is the pre-peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/full/10.1002/nau.23715], which has been published in final form at [http://dx.doi.org/10.1002/nau.23715]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.; identifier:Neurourology and urodynamics Vol.37 No.8 p.2434-2440 (2018 Nov); identifier:07332467; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3545; identifier:Neurourology and urodynamics, 37(8): 2434-2440
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1002/nau.23715
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3545/1/01%e7%94%b2697%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3545/2/02%e7%94%b2697%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

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