A Sensitive and Time-Saving Method for the Diagnosis of Drowning by Multiplex PCR.

• Material Type: 博士論文
• Title: A Sensitive and Time-Saving Method for the Diagnosis of Drowning by Multiplex PCR.
• Author/Editor: Nakanishi, Mari; Nakayama, Akifumi; Kasuda, Shogo; Kudo, Risa; Yuui, Katsuya; Ishitani, Akiko; Hatake, Katsuhiko
• Author Heading: Nakanishi, Mari; Nakayama, Akifumi; Kasuda, Shogo; Kudo, Risa; Yuui, Katsuya; Ishitani, Akiko; Hatake, Katsuhiko
• Publication, Distribution, etc.: 奈良医学会
• Publication Date: 2018-12-31
• Publication Date (W3CDTF): 2018-12-31
• Alternative Title: Multiplex PCRを用いた簡便で感度の高い溺死診断法の開発
• Periodical title: Journal of Nara Medical Association
• No. or year of volume/issue: 69 4,5,6
• Volume: 69
• Issue: 4,5,6
• Pages: 77-85
• ISSN (Periodical Title): ISSN : 1345-0069
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2019-03-15
• Dissertation Number: 乙第1428号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Diagnosis of drowning; Multiplex PCR; Aquatic bacteria; Respiratory tract bacteria
• Target Audience: 一般
• Note (General): type:Thesis; For diagnosing death due to drowning, the method of acid digestion of diatoms is widely used to detect plankton in the organs of the corpse. However, the method is limited by its being complex, hazardous, time-consuming, and insufficiently sensitive. We therefore, developed a novel simple method to diagnose death due to drowning, and determined the location of drowning by detecting genes of representative bacteria in the environment. To procure all the information in one step, the multiplex PCR method was designed. For the diagnosis of drowning, the genes of upper respiratory indigenous bacteria, Streptococcus salivarius and Streptococcus sanguinis were used as indicators. For detection of the location of drowning, Aeromonas hydrophila and Microcystis aeruginosa were used as indicators of freshwater, and Vibrio harveyi as an indicator of seawater. A set of primers was designed for multiplex PCR. to amplify all the bacterial genes simultaneously. Using this method, 47 cases of drowning were examined, and the causes and locations of death were diagnosed.; 博士（医学）・乙第1428号・平成31年3月15日; identifier:Journal of Nara Medical Association Vol.69 No.4,5,6 p.77-85 (2018.12); identifier:13450069; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3560; identifier:Journal of Nara Medical Association, 69(4,5,6): 77-85
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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