γ-Klotho is correlated with resistance to docetaxel in castration-resistant prostate cancer.

• Material Type: 博士論文
• Title: γ-Klotho is correlated with resistance to docetaxel in castration-resistant prostate cancer.
• Author/Editor: Onishi, Kenta; Miyake, Makito; Hori, Shunta; Onishi, Sayuri; Iida, Kota; Morizawa, Yosuke; Tatsumi, Yoshihiro; Nakai, Yasushi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Author Heading: Onishi, Kenta; Miyake, Makito; Hori, Shunta; Onishi, Sayuri; Iida, Kota; Morizawa, Yosuke; Tatsumi, Yoshihiro; Nakai, Yasushi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Publication, Distribution, etc.: Spandidos Publications
• Publication Date: 2020-03
• Publication Date (W3CDTF): 2020-03
• Alternative Title: 去勢抵抗性前立腺癌におけるKlothoγのドセタキセル抵抗性との関連と新規治療としての可能性
• Periodical title: Oncology letters
• No. or year of volume/issue: 19 3
• Volume: 19
• Issue: 3
• Pages: 2306-2316
• ISSN (Periodical Title): ISSN : 1792-1074
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-03-16
• Dissertation Number: 甲第740号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: KLG; CRPC; DTX; OS; xenograft model; PC‑3
• Target Audience: 一般
• Note (General): type:Thesis; The Klotho (KL) gene was first identified as a potent aging suppressor. The KL family currently comprises of three proteins: α-Klotho (KLA), β-Klotho (KLB), and γ-Klotho (KLG). Many studies have shown that KLA and KLB participate in tumor progression or suppression, depending on the type of cancer; however, the relationship between KLG and prostate cancer has not yet been studied. Some studies have claimed that KL is correlated to sensitivity to chemotherapy. Here, we investigated the oncogenic potential of KLG in castration-resistant prostate cancer (CRPC). Immunohistochemical analysis using prostate biopsy specimens revealed that patients with high KLG expression in primary prostate cancer tissue had a significantly poor prognosis for overall survival. In addition, the prostate-specific antigen response rate after docetaxel (DTX) therapy in patients with high KLG expression was lower than that in patients with low KLG expression. To evaluate the potential of KLG as a therapeutic target in human prostate cancer, we generated a xenograft model of human CRPC cell line (PC-3) in male athymic mice. The animals were randomly divided into four groups as follows: i) control group (vehicle only); ii) DTX group (intraperitoneal administration); iii) small interfering RNA targeting KLG (KLG siRNA) group (intratumoral administration); and iv) a combination group (DTX plus KLG siRNA). After 3 weeks of treatment, the tumor weight and tumor Ki-67 labeling index were significantly lower in the KLG siRNA group and the combination group than in the control group. Sensitivity to DTX was increased upon treatment with KLG siRNA. These findings suggest that KLG expression in primary prostate cancer lesions is associated with resistance to DTX in CRPC and has potential as a diagnostic and therapeutic target for patients with CRPC.; 博士（医学）・甲第740号・令和2年3月16日; Copyright: © Onishiet al. This is an open access article distributed under theterms of CreativeCommons Attribution License(https://creativecommons.org/licenses/by-nc-nd/4.0/).; identifier:Oncology letters Vol.19 No.3 p.2306-2316 (2020 Mar); identifier:17921074; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3734; identifier:Oncology letters, 19(3): 2306-2316
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3892/ol.2020.11308
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3734/1/01%e7%94%b2740%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3734/2/02%e7%94%b2740%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3734/3/03%e7%94%b2740%e6%9c%ac%e6%96%87.pdf fulltext