Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma.

• Material Type: 博士論文
• Title: Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma.
• Author/Editor: Iida, Kota; Miyake, Makito; Onishi, Kenta; Hori, Shunta; Morizawa, Yosuke; Gotoh, Daisuke; Itami, Yoshitaka; Onishi, Sayuri; Nakai, Yasushi; Anai, Satoshi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Author Heading: Iida, Kota; Miyake, Makito; Onishi, Kenta; Hori, Shunta; Morizawa, Yosuke; Gotoh, Daisuke; Itami, Yoshitaka; Onishi, Sayuri; Nakai, Yasushi; Anai, Satoshi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• Publication, Distribution, etc.: Spandidos Publications
• Publication Date: 2019-04
• Publication Date (W3CDTF): 2019-04
• Alternative Title: 限局性腎細胞癌における腫瘍浸潤性リンパ球と関連サイトカインの予後因子としての意義
• Periodical title: Oncology letters
• No. or year of volume/issue: 17 4
• Volume: 17
• Issue: 4
• Pages: 4004-4010
• ISSN (Periodical Title): ISSN : 1792-1074
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-03-16
• Dissertation Number: 甲第740号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: renal cell carcinoma; cluster of differentiation 276; Foxp3; tumor‑infiltrating lymphocyte; cytokine
• Target Audience: 一般
• Note (General): type:Thesis; Renal cell carcinoma (RCC) is an immunogenic tumor and pathological specimen generally contain large quantities of tumor-infiltrating lymphocytes (TILs). Numerous cell types and cytokines could affect the immune escape mechanism of tumor cells. The aim of the present study was to investigate the prognostic impact of TILs and the associated circulating cytokines on localized clear cell RCC following radical nephrectomy. A total of 87 patients who had undergone radical nephrectomy and were pathologically diagnosed with localized clear cell RCC were included. The present study evaluated the profile of TILs with immunohistochemical analysis of tumor specimens using a panel of antibodies [cluster of differentiation (CD)-4, CD8, CD80, CD86, CD276, and Forkhead box p3 (Foxp3)]. Counts of each TIL were compared with clinicopathological variables. Based on the results of immunohistochemical analyses, putative cytokines, including interleukin (IL)-6, IL-10, IL-17, interferon-γ, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β, were selected, and their levels in preoperative serum were measured by ELISA. The levels were compared with TIL counts in tumor specimens. High counts of the CD276+ and Foxp3+ TILs were identified as independent factors for poor prognosis for metastasis and local recurrence following radical nephrectomy (P=0.033 and 0.006, respectively). A high CD276+ TIL count was associated with preoperative serum levels of TNF-α and IFN-γ (P=0.027 and P=0.035, respectively), whereas a high count of Foxp3+ TILs was associated with preoperative serum levels of TGF-β (P=0.021). High levels of TNF-α and TGF-β were associated with recurrence-free survival (P=0.035 and P=0.031, respectively). Topical intra-tumoral immunoreaction and systemic immune status may be associated with patients with localized RCC. The topical induction of the CD276+ and Foxp3+ TILs was suggested to be associated with high levels of serum TNF-α and IFN-γ. Preoperative serum levels of TNF-α and TGF-β could be simple and non-invasive biomarkers for risk stratification before radical surgery.; 博士（医学）・甲第741号・令和2年3月16日; Copyright © Spandidos Publications 2019. All rights reserved.; Articles from Oncology Letters are provided here courtesy of Spandidos Publications.; identifier:Oncology letters Vol.17 No.4 p.4004-4010 (2019 Apr); identifier:17921074; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3735; identifier:Oncology letters, 17(4): 4004-4010
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3735/1/01%e7%94%b2741%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3735/2/02%e7%94%b2741%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3735/3/03%e7%94%b2741%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3892/ol.2019.10057
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU