Differentiation of embryonic stem cells into inner ear vestibular hair cells using vestibular cell derived-conditioned medium.

• Material Type: 博士論文
• Title: Differentiation of embryonic stem cells into inner ear vestibular hair cells using vestibular cell derived-conditioned medium.
• Author/Editor: Sakagami, Masaharu; Ouji, Yukiteru; Kawai, Norikazu; Misu, Masayasu; Yoshikawa, Masahide; Kitahara, Tadashi
• Author Heading: Sakagami, Masaharu; Ouji, Yukiteru; Kawai, Norikazu; Misu, Masayasu; Yoshikawa, Masahide; Kitahara, Tadashi
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2019-09
• Publication Date (W3CDTF): 2019-09
• Alternative Title: 内耳前庭細胞由来の培養上清を用いた胚性幹細胞から内耳前庭有毛細胞への分化誘導
• Periodical title: Biochemistry and biophysics reports
• No. or year of volume/issue: 19
• Volume: 19
• Pages: Article No.100649-
• ISSN (Periodical Title): ISSN : 2405-5808
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-03-16
• Dissertation Number: 甲第743号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Embryonic stem cells; Vestibular; Inner ear; Hair cell; Differentiation; Conditioned medium
• Target Audience: 一般
• Note (General): type:Thesis; Vestibular hair cells (V-HCs) in the inner ear have important roles and various functions. When V-HCs are damaged, crippling symptoms, such as vertigo, visual field oscillation, and imbalance, are often seen. Recently, several studies have reported differentiation of embryonic stem (ES) cells, as pluripotent stem cells, to HCs, though a method for producing V-HCs has yet to be established. In the present study, we used vestibular cell conditioned medium (V-CM) and effectively induced ES cells to differentiate into V-HCs. Expressions of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5) were significantly increased in ES cells cultured in V-CM for 2 weeks, while those were not observed in ES cells cultured without V-CM. On the other hand, the cochlear HC-related marker Lmod3 was either not detected or detected only faintly in those cells when cultured in V-CM. Our results demonstrate that V-CM has an ability to specifically induce differentiation of ES cells into V-HCs.; 博士（医学）・甲第743号・令和2年3月16日; © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).; identifier:Biochemistry and biophysics reports Vol.19 Article No.100649 (2019 May); identifier:24055808; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3737; identifier:Biochemistry and biophysics reports, 19: Article No.100649
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3737/1/01%e7%94%b2743%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3737/2/02%e7%94%b2743%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3737/3/03%e7%94%b2743%e6%9c%ac%e6%96%87.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3737/4/04%e7%94%b2743Supplementary%20data.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.bbrep.2019.100649
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU