Immunohistochemical expression of CD44v9 and 8-OHdG in ovarian endometrioma and the benign endometriotic lesions adjacent to clear cell carcinoma.

• Material Type: 博士論文
• Title: Immunohistochemical expression of CD44v9 and 8-OHdG in ovarian endometrioma and the benign endometriotic lesions adjacent to clear cell carcinoma.
• Author/Editor: Niiro, Emiko; Kawahara, Naoki; Yamada, Yuki; Yoshimoto, Chiharu; Shimada, Keiji; Sudo, Tamotsu; Kobayashi, Hiroshi
• Author Heading: Niiro, Emiko; Kawahara, Naoki; Yamada, Yuki; Yoshimoto, Chiharu; Shimada, Keiji; Sudo, Tamotsu; Kobayashi, Hiroshi
• Publication, Distribution, etc.: Wiley
• Publication Date: 2019-11
• Publication Date (W3CDTF): 2019-11
• Alternative Title: 卵巣子宮内膜症性嚢胞の癌化におけるCD44v9と8-OHdGの発現
• Periodical title: The journal of obstetrics and gynaecology research
• No. or year of volume/issue: 45 11
• Volume: 45
• Issue: 11
• Pages: 2260-2266
• ISSN (Periodical Title): ISSN : 1341-8076
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-03-16
• Dissertation Number: 乙第1452号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: 8-OHdG; CD44v9; clear cell carcinoma of the ovary; endometriosis; immunohistochemistry
• Target Audience: 一般
• Note (General): type:Thesis; Aim: Expression of CD44 variant isoforms (CD44v) promotes the synthesis of reduced glutathione and contributes to reactive oxygen species defense through up-regulation of the intracellular antioxidant. The aim of the study was to investigate the expression of CD44v9 and oxidative DNA damage marker, 8-OHdG, in benign ovarian endometrioma (OE) and OE harboring clear cell carcinomas (CCC). Methods: A retrospective study was performed at the Department of Gynecology, Nara Medical University hospital from January 2006 to December 2012. Patients with histologically confirmed benign OE (n = 27) and OE harboring areas of CCC (n = 8) were selected. Tissue samples were immunohistochemically analyzed for the presence of CD44v9 and 8-OHdG using avidin-biotin complex method. Results: CD44v9 was located on the cell membrane of endometriotic epithelial cells and expressed in 88.9% (24/27) of benign OE tissues. Only 25.0% (2/8) of benign endometriotic lesions adjacent to CCC was found to stain weakly for CD44v9. Percentage of CD44v9 positive cells was 68.5 ± 20.2% (mean ± standard deviation) of benign OE, 16.7 ± 16.5% of CCC endometriotic tissue (P < 0.001). Compared to benign OE, CCC endometriotic tissue showed a significant increase in the proportion of 8-OHdG expression (77.3 ± 22.5% vs 94.9 ± 3.0%, P = 0.049). A significant negative correlation was observed between CD44v9 status and 8-OHdG nuclear expression (r = -0.458, P = 0.006). Conclusion: Alterations in CD44v9 and 8-OHdG may be associated with malignant transformation of benign OE.; 博士（医学）・乙第1452号・令和2年3月16日; © 2019 Japan Society of Obstetrics and Gynecology.; This is the peer reviewed version of the following article: [https://obgyn.onlinelibrary.wiley.com/doi/full/10.1111/jog.14093], which has been published in final form at [https://doi.org/10.1111/jog.14093]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.; identifier:The journal of obstetrics and gynaecology research Vol.45 No.11 p.2260-2266 (2019 Nov); identifier:13418076; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3742; identifier:The journal of obstetrics and gynaecology research, 45(11): 2260-2266
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1111/jog.14093
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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