Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes.

• Material Type: 博士論文
• Title: Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes.
• Author/Editor: Ogawa, Yoshihiko; Nakano, Ryuichi; Kasahara, Kei; Mizuno, Tomoki; Hirai, Nobuyasu; Nakano, Akiyo; Suzuki, Yuki; Kakuta, Naoki; Masui, Takashi; Yano, Hisakazu; Mikasa, Keiichi
• Author Heading: Ogawa, Yoshihiko; Nakano, Ryuichi; Kasahara, Kei; Mizuno, Tomoki; Hirai, Nobuyasu; Nakano, Akiyo; Suzuki, Yuki; Kakuta, Naoki; Masui, Takashi; Yano, Hisakazu; Mikasa, Keiichi
• Publication, Distribution, etc.: Public Library of Science
• Publication Date: 2019-11-13
• Publication Date (W3CDTF): 2019-11-13
• Alternative Title: プラスミド性キノロン耐性遺伝子を保有するIMP-6産生腸内細菌科細菌でのイノカラムサイズ効果の比較
• Periodical title: PloS one
• No. or year of volume/issue: 14 11
• Volume: 14
• Issue: 11
• Pages: Article No.e0225210-
• ISSN (Periodical Title): ISSN : 1932-6203
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-06-30
• Dissertation Number: 乙第1463号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:Thesis; Almost all cases of carbapenemase-producing Enterobacteriaceae infections in Japan are caused by blaIMP-positive Enterobacteriaceae (especially blaIMP-6) and infections caused by other types of carbapenemase-producing Enterobacteriaceae are quite rare. We examined drug resistance genes co-harboring with blaIMP-6 and their inoculum size effects. We screened β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes, and aminoglycoside-modifying enzyme genes by PCR and performed sequencing for 14 blaIMP-6-positive Enterobacteriaceae. Further, all PMQR-positive isolates were submitted to conjugation and inoculum effect evaluation. Our data showed that 13 of the 14 isolates harbored CTX-M-2 and one co-harbored CTX-M-2 and CTX-M-1 as extended-spectrum β-lactamases. All isolates carried one or more PMQRs; aac(6’)-Ib-cr was the most prevalent (92.8%), and was followed by oqxA (64.3%), qnrS (50%), oqxAB (21.4%), and qnrB (14.3%). However, Klebsiella pneumoniae contains chromosomal OqxAB. Inoculum size effects were significant in all strains for meropenem, 13 strains for imipenem, 7 for levofloxacin, and 3 for amikacin. We observed that 11 of the experimental strains (100%), 8 strains (72.7%), and 1 strain showed inoculum size effects for meropenem, imipenem, and amikacin, respectively. However, four strains harbored qnr genes and two strains harbored qnr genes and QRDR mutations concurrently; no inoculum size effect was seen for levofloxacin. The blaIMP-6-positive Enterobacteriaceae that we studied was found to harbor at least one plasmid-mediated drug resistance gene. The inoculum size effect for carbapenems was thought to be mainly due to IMP-6-type metallo-β-lactamase; however qnrB and qnrS also had a minimal impact on the inoculum size effect for levofloxacin.; 博士（医学）・乙第1463号・令和2年6月30日; Copyright: © 2019 Ogawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PloS one Vol.14 No.11 Article No.e0225210 (2019 Nov); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3764; identifier:PloS one, 14(11): Article No.e0225210
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3764/1/01%e4%b9%991463%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3764/2/02%e4%b9%991463%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3764/3/03%e4%b9%991463%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1371/journal.pone.0225210
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU