K-ras mutation analysis of residual liquid-based cytology specimens from endoscopic ultrasound-guided fine needle aspiration improves cell block diagnosis of pancreatic ductal adenocarcinoma.

• Material Type: 博士論文
• Title: K-ras mutation analysis of residual liquid-based cytology specimens from endoscopic ultrasound-guided fine needle aspiration improves cell block diagnosis of pancreatic ductal adenocarcinoma.
• Author/Editor: Sekita-Hatakeyama, Yoko; Nishikawa, Takeshi; Takeuchi, Mao; Morita, Kouhei; Takeda, Maiko; Hatakeyama, Kinta; Nakai, Tokiko; Uchiyama, Tomoko; Itami, Hiroe; Fujii, Tomomi; Mitoro, Akira; Sho, Masayuki; Ohbayashi, Chiho
• Author Heading: Sekita-Hatakeyama, Yoko; Nishikawa, Takeshi; Takeuchi, Mao; Morita, Kouhei; Takeda, Maiko; Hatakeyama, Kinta; Nakai, Tokiko; Uchiyama, Tomoko; Itami, Hiroe; Fujii, Tomomi; Mitoro, Akira; Sho, Masayuki; Ohbayashi, Chiho
• Publication, Distribution, etc.: Public Library of Science
• Publication Date: 2018-03-01
• Publication Date (W3CDTF): 2018-03-01
• Alternative Title: 内視鏡超音波ガイド下穿刺吸引の液状検体の残余を用いたK-ras 遺伝子検査は正診率を高める
• Periodical title: PloS one
• No. or year of volume/issue: 13 3
• Volume: 13
• Issue: 3
• Pages: Article No.e0193692-
• ISSN (Periodical Title): ISSN : 1932-6203
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2020-12-24
• Dissertation Number: 乙第1492号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:Thesis; Background: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) technology is widely used for the diagnosis of pancreatic masses. However, in some cases, inadequate tissue volume or difficulty of morphological diagnosis are constraining factors for adequate cytopathological evaluation. K-ras mutation is the most frequently acquired genetic abnormality, occurring in approximately 90% of all patients with pancreatic ductal adenocarcinoma (PDAC). In the present study, the clinical utility of residual liquid-based cytology (LBC) specimens obtained using EUS-FNA for K-ras mutation analysis was evaluated. Methods: In this study, 81 patients with pancreatic lesions were examined. The cell block (CB) specimens separated from EUS-FNA samples were morphologically evaluated by hematoxylin-eosin (HE) staining. Final diagnoses were confirmed by CB specimens, surgical resection specimens, diagnostic imaging, and clinical follow-up. Genomic DNA of residual LBC specimens stored at 4°C for several months were extracted and assessed for K-ras mutations using a fluorescence resonance energy transfer-based preferential homoduplex formation assay. Results: K-ras mutation analysis using residual LBC samples was successful in all cases. The sensitivity, specificity, and accuracy of CB examination alone were 77.4%, 100%, and 81.3%, respectively, and those of the combination of CB examination and K-ras mutation analysis were 90.3%, 92.3%, and 90.7%, respectively. Furthermore, K-ras mutations were detected in 8 (57.1%) of 14 PDAC samples for which the CB results were inconclusive. Conclusion: These findings suggest that K-ras mutation analysis using residual LBC specimens improves the diagnostic accuracy of EUS-FNA.; 博士（医学）・乙第1492号・令和2年12月24日; Copyright: © 2018 Sekita-Hatakeyama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PloS one Vol.13 No.3 Article No.e0193692 (2018 Mar); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3837; identifier:PloS one, 13(3): Article No.e0193692
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3837/1/01%e4%b9%991492%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3837/2/02%e4%b9%991492%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3837/3/03%e4%b9%991492%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1371/journal.pone.0193692
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU