Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder.

• Material Type: 博士論文
• Title: Disabled Homolog 2 (DAB2) Protein in Tumor Microenvironment Correlates with Aggressive Phenotype in Human Urothelial Carcinoma of the Bladder.
• Author/Editor: Itami, Yoshitaka; Miyake, Makito; Ohnishi, Sayuri; Tatsumi, Yoshihiro; Gotoh, Daisuke; Hori, Shunta; Morizawa, Yosuke; Iida, Kota; Ohnishi, Kenta; Nakai, Yasushi; Inoue, Takeshi; Anai, Satoshi; Tanaka, Nobumichi; Fujii, Tomomi; Shimada, Keiji; Furuya, Hideki; Khadka, Vedbar S; Deng, Youping; Fujimoto, Kiyohide
• Author Heading: Itami, Yoshitaka; Miyake, Makito; Ohnishi, Sayuri; Tatsumi, Yoshihiro; Gotoh, Daisuke; Hori, Shunta; Morizawa, Yosuke; Iida, Kota; Ohnishi, Kenta; Nakai, Yasushi; Inoue, Takeshi; Anai, Satoshi; Tanaka, Nobumichi; Fujii, Tomomi; Shimada, Keiji; Furuya, Hideki; Khadka, Vedbar S; Deng, Youping; Fujimoto, Kiyohide
• Publication, Distribution, etc.: MDPI
• Publication Date: 2020-01-20
• Publication Date (W3CDTF): 2020-01-20
• Alternative Title: 尿路上皮癌微小環境内におけるDisabled Homolog 2 (DAB2) は腫瘍細胞上皮間葉転換を介して遊走能・浸潤能を高める
• Periodical title: Diagnostics
• No. or year of volume/issue: 10 1
• Volume: 10
• Issue: 1
• Pages: Article No.54-
• ISSN (Periodical Title): ISSN : 2075-4418
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2021-03-15
• Dissertation Number: 甲第768号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: DAB2; bladder cancer; epithelial-mesenchymal transition
• Target Audience: 一般
• Note (General): type:Thesis; Disabled homolog-2 (DAB2) has been reported to be a tumor suppressor gene. However, a number of contrary studies suggested that DAB2 promotes tumor invasion in urothelial carcinoma of the bladder (UCB). Here, we investigated the clinical role and biological function of DAB2 in human UCB. Immunohistochemical staining analysis for DAB2 was carried out on UCB tissue specimens. DAB2 expression levels were compared with clinicopathological factors. DAB2 was knocked-down by small interfering RNA (siRNA) transfection, and then its effects on cell proliferation, invasion, and migration, and changes to epithelial-mesenchymal transition (EMT)-related proteins were evaluated. In our in vivo assays, tumor-bearing athymic nude mice subcutaneously inoculated with human UCB cells (MGH-U-3 or UM-UC-3) were treated by DAB2-targeting siRNA. Higher expression of DAB2 was associated with higher clinical T category, high tumor grade, and poor oncological outcome. The knock-down of DAB2 decreased both invasion and migration ability and expression of EMT-related proteins. Significant inhibitory effects on tumor growth and invasion were observed in xenograft tumors of UM-UC-3 treated by DAB2-targeting siRNA. Our findings suggested that DAB2 expression was associated with poor prognosis through increased oncogenic properties including tumor proliferation, migration, invasion, and enhancement of EMT in human UCB.; 博士（医学）・甲第768号・令和3年3月15日; © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).; identifier:Diagnostics Vol.10 No.1 Article No.54 (2020 Jan); identifier:20754418; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3892; identifier:Diagnostics, 10(1): Article No.54
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3892/1/01%e7%94%b2768%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3892/2/02%e7%94%b2768%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3892/3/03%e7%94%b2768%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3390/diagnostics10010054
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU