Clinical characteristics and molecular epidemiology of invasive Streptococcus agalactiae infections between 2007 and 2016 in Nara, Japan.

• Material Type: 博士論文
• Title: Clinical characteristics and molecular epidemiology of invasive Streptococcus agalactiae infections between 2007 and 2016 in Nara, Japan.
• Author/Editor: Hirai, Nobuyasu; Kasahara, Kei; Nakano, Ryuichi; Ogawa, Yoshihiko; Suzuki, Yuki; Ogawa, Miho; Hishiya, Naokuni; Nakano, Akiyo; Ichimura, Sadahiro; Yano, Hisakazu; Yoshikawa, Masahide
• Author Heading: Hirai, Nobuyasu; Kasahara, Kei; Nakano, Ryuichi; Ogawa, Yoshihiko; Suzuki, Yuki; Ogawa, Miho; Hishiya, Naokuni; Nakano, Akiyo; Ichimura, Sadahiro; Yano, Hisakazu; Yoshikawa, Masahide
• Publication, Distribution, etc.: Public Library of Science
• Publication Date: 2020-10-19
• Publication Date (W3CDTF): 2020-10-19
• Alternative Title: 奈良における侵襲性GBS感染症における臨床的特徴と分子疫学的特徴(2007～2016年)
• Periodical title: PloS one
• No. or year of volume/issue: 15 10
• Volume: 15
• Issue: 10
• Pages: Article No.e0240590-
• ISSN (Periodical Title): ISSN : 1932-6203
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2021-03-15
• Dissertation Number: 甲第773号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:Thesis; Invasive Streptococcus agalactiae (GBS) infections are increasingly common among neonates and the elderly. Therefore, GBS surveillance for better antibiotic treatment and prophylaxis strategies are needed. We retrospectively evaluated the clinical aspects of invasive infections and the phenotypic and genetic diversity of infectious isolates from Nara, Japan, collected between 2007 and 2016, by using information from hospital records. GBS strains collected from the blood and cerebrospinal fluid cultures were evaluated for capsular types, multi-locus sequence typing (MLST), antibiotic susceptibility, antibiotics resistance gene, and pulsed-field gel electrophoresis. Forty GBS isolates (10 from children and 30 from adults) were analyzed, and the distribution of molecular serotype and allelic profiles varied between children and adults. We found the rates of early-onset disease in neonates with birth complications to be higher than that of previous reports, indicating that there could be relevance between complications at birth and early-onset disease. Standard antibiotic prophylaxis strategies may need to be reconsidered in patients with birth complications. In adults, the mean age of the patients was 68 years (male: 63%). Primary bacteremia was the most common source of infection. In the neonates, six had early-onset diseases and four had late-onset diseases. The most frequently identified strains were molecular serotype Ia ST23 (40%) and molecular serotype Ib ST10 (20%) in children and molecular serotype Ib ST10 (17%), molecular serotype VI ST1 (13%), and molecular serotype V ST1 (13%) in adults. Levofloxacin-resistant molecular serotype Ib strains and molecular serotypes V and VI ST1 were common causes of GBS infection in adults but were rarely found in children. Furthermore, pulsed-field gel electrophoresis in our study showed that specific clone isolates, that tend to have antibiotics resistance were widespread horizontally for a decade. Continuous surveillance and molecular investigation are warranted to identify the transmission route and improve antibiotic treatment strategies.; 博士（医学）・甲第773号・令和3年3月15日; Copyright: © 2020 Hirai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PloS one Vol.15 No.10 Article No.e0240590 (2020 Oct); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3897; identifier:PloS one, 15(10): Article No.e0240590
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1371/journal.pone.0240590
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3897/1/01%e7%94%b2773%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3897/2/02%e7%94%b2773%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3897/4/04%e7%94%b2773Suppl..pdf fulltext