Prevalence and Relatedness of mcr-1-Mediated Colistin-Resistant Escherichia coli Isolated From Livestock and Farmers in Japan.

• Material Type: 博士論文
• Title: Prevalence and Relatedness of mcr-1-Mediated Colistin-Resistant Escherichia coli Isolated From Livestock and Farmers in Japan.
• Author/Editor: Nakano, Akiyo; Nakano, Ryuichi; Nishisouzu, Ryuji; Suzuki, Yuki; Horiuchi, Saori; Kikuchi-Ueda, Takane; Ubagai, Tsuneyuki; Ono, Yasuo; Yano, Hisakazu
• Author Heading: Nakano, Akiyo; Nakano, Ryuichi; Nishisouzu, Ryuji; Suzuki, Yuki; Horiuchi, Saori; Kikuchi-Ueda, Takane; Ubagai, Tsuneyuki; Ono, Yasuo; Yano, Hisakazu
• Publication, Distribution, etc.: Frontiers Media
• Publication Date: 2021-04-26
• Publication Date (W3CDTF): 2021-04-26
• Alternative Title: 日本の家畜と畜産農家から分離した mcr-1 によるコリスチン耐性大腸菌の分布状況と関連性について
• Periodical title: Frontiers in microbiology
• No. or year of volume/issue: 12
• Volume: 12
• Pages: Article No.664931-
• ISSN (Periodical Title): ISSN : 1664-302X
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2021-09-29
• Dissertation Number: 甲第798号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: colistin resistance; mcr-1; Escherichia coli; livestock; farmer; genotype; one health
• Target Audience: 一般
• Note (General): type:Thesis; Colistin is used to treat infectious diseases in humans and livestock; it has also been used as a feed additive for livestock for approximately 50 years. Since the mcr-1 plasmid-mediated colistin resistance gene was discovered in China in 2015, it has been detected worldwide, mainly in livestock. In this study, we investigated the prevalence and characteristics of mcr-mediated colistin-resistant Escherichia coli in livestock and farmers in Japan. We collected fecal samples from 295 healthy livestock (202 cattle and 93 swine) and 62 healthy farmers from 72 livestock farms (58 cattle farms and 14 swine farms) between 2013 and 2015. Twenty-eight mcr-1-harboring E. coli strains were isolated from 25 livestock (six cattle and 19 swine) and three farmers (two cattle farmers and one swine farmer). The prevalence rates of mcr-1-harboring E. coli in livestock and farmers were 8.47 and 4.84%, respectively. Of the 28 strains, the resistance genes of three were transferable via the mcr-1-coding plasmids to E. coli J53 at low frequencies (10-7-10-8). Six strains coharbored mcr-1 with CTX-M β-lactamases (CTX-M-14, CTX-M-27, or CTX-M-156). Of the isolates obtained from livestock and farmers in four farms (farms C, I, N, and P), nine strains had the same genotypical characteristics (sequence types and pulsed-field gel electrophoresis band patterns), plasmid characteristics (incompatibility group and plasmid transferability), and minimum inhibitory concentrations. Thus, the findings suggested that clonal strains could spread among livestock and farmers within farms. To our knowledge, this is the first study to detect clonal relatedness of mcr-1-mediated colistin-resistant E. coli in livestock and farmers. It is suggested that farmers are at a higher risk of acquiring mcr-1-harboring strains, calling for our attention based on the One Health concept.; 博士（医学）・甲第798号・令和3年9月29日; Copyright © 2021 Nakano, Nakano, Nishisouzu, Suzuki, Horiuchi, Kikuchi-Ueda, Ubagai, Ono and Yano. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.; identifier:Frontiers in microbiology Vol.12 Article No.664931 (2021 Apr); identifier:1664302X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3947; identifier:Frontiers in microbiology, 12: Article No.664931
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3947/1/01%e7%94%b2798%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3947/2/02%e7%94%b2798%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3947/3/03%e7%94%b2798%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3389/fmicb.2021.664931
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU