Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.

• Material Type: 博士論文
• Title: Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.
• Author/Editor: Fujioka, Nobuhiro; Kitabatake, Masahiro; Ouji-Sageshima, Noriko; Ibaraki, Takahiro; Kumamoto, Makiko; Fujita, Yukio; Hontsu, Shigeto; Yamauchi, Motoo; Yoshikawa, Masanori; Muro, Shigeo; Ito, Toshihiro
• Author Heading: Fujioka, Nobuhiro; Kitabatake, Masahiro; Ouji-Sageshima, Noriko; Ibaraki, Takahiro; Kumamoto, Makiko; Fujita, Yukio; Hontsu, Shigeto; Yamauchi, Motoo; Yoshikawa, Masanori; Muro, Shigeo; Ito, Toshihiro
• Publication, Distribution, etc.: DOVE Medical Press
• Publication Date: 2021-10-08
• Publication Date (W3CDTF): 2021-10-08
• Alternative Title: ヒト脂肪由来間葉型幹細胞は、間葉上皮転換によりエラスターゼ誘発マウス肺気腫を改善する
• Periodical title: International journal of chronic obstructive pulmonary disease
• No. or year of volume/issue: 16
• Volume: 16
• Pages: 2783-2793
• ISSN (Periodical Title): ISSN : 1176-9106
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2022-03-15
• Dissertation Number: 甲第825号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: chronic obstructive pulmonary disease; mesenchymal–epithelial transition; adipose derived mesenchymal stem cell; pulmonary function test
• Target Audience: 一般
• Note (General): type:Thesis; Purpose: Chronic obstructive pulmonary disease (COPD) is a worldwide problem because of its high prevalence and mortality. However, there is no fundamental treatment to ameliorate their pathological change in COPD lung. Recently, adipose-derived mesenchymal stem cells (ADSCs) have attracted attention in the field of regenerative medicine to repair damaged organs. Moreover, their utility in treating respiratory diseases has been reported in some animal models. However, the detailed mechanism by which ADSCs improve chronic respiratory diseases, including COPD, remains to be elucidated. We examined whether human ADSCs (hADSCs) ameliorated elastase-induced emphysema and whether hADSCs differentiated into alveolar epithelial cells in a murine model of COPD. Methods: Female SCID-beige mice (6 weeks old) were divided into the following four groups according to whether they received an intratracheal injection of phosphate-buffered saline or porcine pancreatic elastase, and whether they received an intravenous injection of saline or hADSCs 3 days after intratracheal injection; Control group, hADSC group, Elastase group, and Elastase-hADSC group. We evaluated the lung function, assessed histological changes, and compared gene expression between hADSCs isolated from the lung of Elastase-hADSC group and naïve hADSCs 28 days after saline or elastase administration. Results: hADSCs improved the pathogenesis of COPD, including the mean linear intercept and forced expiratory volume, in an elastase-induced emphysema model in mice. Furthermore, hADSCs were observed in the lungs of elastase-treated mice at 25 days after administration. These cells expressed genes related to mesenchymal-epithelial transition and surface markers of alveolar epithelial cells, such as TTF-1, β-catenin, and E-cadherin. Conclusion: hADSCs have the potential to improve the pathogenesis of COPD by differentiating into alveolar epithelial cells by mesenchymal-epithelial transition.; 博士（医学）・甲第825号・令和4年3月15日; © 2021 Fujioka et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).; identifier:International journal of chronic obstructive pulmonary disease Vol.16 p.2783-2793 (2021 Oct); identifier:11769106; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4008; identifier:International journal of chronic obstructive pulmonary disease, 16: 2783-2793
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4008/1/01%e7%94%b2825%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4008/2/02%e7%94%b2825%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4008/3/03%e7%94%b2825%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2147/COPD.S324952
• Periodical Title (NCID): AA12158595; https://ci.nii.ac.jp/ncid/AA12158595
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU