Intermittent Hypoxia Upregulates the Renin and Cd38 mRNAs in Renin-Producing Cells via the Downregulation of miR-203.

• Material Type: 博士論文
• Title: Intermittent Hypoxia Upregulates the Renin and Cd38 mRNAs in Renin-Producing Cells via the Downregulation of miR-203.
• Author/Editor: Takeda, Yoshinori; Itaya-Hironaka, Asako; Yamauchi, Akiyo; Makino, Mai; Sakuramoto-Tsuchida, Sumiyo; Ota, Hiroyo; Kawaguchi, Ryuji; Takasawa, Shin
• Author Heading: Takeda, Yoshinori; Itaya-Hironaka, Asako; Yamauchi, Akiyo; Makino, Mai; Sakuramoto-Tsuchida, Sumiyo; Ota, Hiroyo; Kawaguchi, Ryuji; Takasawa, Shin
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-09-19
• Publication Date (W3CDTF): 2021-09-19
• Alternative Title: 間歇的低酸素によりレニン産生細胞では microRNA-203 の発現低下を介して Renin と Cd38 の発現上昇が起こる
• Periodical title: International journal of molecular sciences
• No. or year of volume/issue: 22 18
• Volume: 22
• Issue: 18
• Pages: Article No.10127-
• ISSN (Periodical Title): ISSN : 1422-0067
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2022-03-15
• Dissertation Number: 甲第831号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: CD38; cyclic ADP-ribose; intermittent hypoxia; juxtaglomerular cell; miR-203; renin-angiotensin system; sleep apnea syndrome
• Target Audience: 一般
• Note (General): type:Thesis; Sleep apnea syndrome is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), and it is a known risk factor for hypertension. The upregulation of the renin-angiotensin system has been reported in IH, and the correlation between renin and CD38 has been noted. We exposed human HEK293 and mouse As4.1 renal cells to experimental IH or normoxia for 24 h and then measured the mRNA levels using a real-time reverse transcription polymerase chain reaction. The mRNA levels of Renin (Ren) and Cd38 were significantly increased by IH, indicating that they could be involved in the CD38-cyclic ADP-ribose signaling pathway. We next investigated the promotor activities of both genes, which were not increased by IH. Yet, a target mRNA search of the microRNA (miRNA) revealed both mRNAs to have a potential target sequence for miR-203. The miR-203 level of the IH-treated cells was significantly decreased when compared with the normoxia-treated cells. The IH-induced upregulation of the genes was abolished by the introduction of the miR-203 mimic, but not the miR-203 mimic NC negative control. These results indicate that IH stress downregulates the miR-203 in renin-producing cells, thereby resulting in increased mRNA levels of Ren and Cd38, which leads to hypertension.; 博士（医学）・甲第831号・令和4年3月15日; © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).; identifier:International journal of molecular sciences Vol.22 No.18 Article No.10127 (2021 Sep); identifier:14220067; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4014; identifier:International journal of molecular sciences, 22(18): Article No.10127
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4014/1/01%e7%94%b2831%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4014/2/02%e7%94%b2831%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4014/3/03%e7%94%b2831%e6%9c%ac%e6%96%87.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3390/ijms221810127
• Periodical Title (NCID): AA12038549; https://ci.nii.ac.jp/ncid/AA12038549
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU