Neuroprotective effects of pravastatin in cerebral venous infarction in a rat model

• Material Type: 博士論文
• Title: Neuroprotective effects of pravastatin in cerebral venous infarction in a rat model
• Author/Editor: Sato, Fumiya; Wajima, Daisuke; Takeshima, Yasuhiro; Nakagawa, Ichiro; Kim, Taekyun; Motoyama, Yasushi; Park, Young-Soo; Nakase, Hiroyuki
• Author Heading: Sato, Fumiya; Wajima, Daisuke; Takeshima, Yasuhiro; Nakagawa, Ichiro; Kim, Taekyun; Motoyama, Yasushi; Park, Young-Soo; Nakase, Hiroyuki
• Publication, Distribution, etc.: Elsevier Ltd.
• Publication Date: 2023-06
• Publication Date (W3CDTF): 2023-06
• Alternative Title: 脳皮質静脈梗塞ラットモデルを用いた脳静脈虚血におけるプラバスタチンの神経保護効果
• Periodical title: IBRO Neuroscience Reports
• No. or year of volume/issue: 14
• Volume: 14
• Pages: 202-209
• ISSN (Periodical Title): ISSN : 2667-2421
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2023-06-23
• Dissertation Number: 甲第885号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Cerebral blood flow; Cerebral cortex; Cerebral ischemia; Neuronal apoptosis; Pravastatin sodium; Vein
• Target Audience: 一般
• Note (General): type:Thesis; Objectives: Pravastatin sodium is reported to have multiple beneficial effects in cerebral atherosclerosis and neuronal injury; however, the preventive effects on cerebral venous ischemia are still unknown. Herein, we aimed to examine the neuroprotective effects of transoral prior administration of pravastatin sodium against cerebral cortical venous ischemia with suppression of apoptosis. Methods: Thirty 8-week-old male Wistar rats were divided equally into two study groups (n = 15 vs. n = 15); the pravastatin group was fed 1% pravastatin sodium with their usual diet for 2 weeks, while the control group only received the usual diet. Two-vein occlusion (2VO) model was applied for this study, and two adjacent cortical veins in each animal were permanently occluded photochemically with rose bengal dye. During photothrombosis, regional changes of the cerebral blood flow (CBF) in area of the venous ischemia were recorded. At 48-h after 2VO, animals were euthanized using perfusion fixation, and we histologically measured ratios of infarcted area to contralateral hemisphere, and counted Bax- and Bcl-2-positive cells in the penumbra to investigate the implications for apoptosis. Results: The ratio of infarcted area was significantly decreased in the pravastatin group compared to the control group (P < 0.01). The number of Bax-positive cells also decreased significantly in the pravastatin group (P < 0.01). In contrast, immunolabeling for Bcl-2 was essentially negative in all areas in both groups. There were also no significant differences in regional CBF changes after 2VO between the two groups (P = 0.13). Conclusions: Pre-emptive administration of pravastatin sodium mixed in the food has neuroprotective effects against cerebral cortical venous ischemia with suppression of apoptosis associated with inhibition of Bax expression but has little influence on regional CBF.; 権利情報：© 2023 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).; identifier:IBRO Neuroscience Reports. 2023 Jun, vol.14, p.202-209; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4207; identifier:IBRO Neuroscience Reports, 14: 202-209
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4207/2/K885%20full.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4207/3/K885_abstract.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4207/4/K885_review.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.ibneur.2023.02.002
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU