Overexpression of microRNA-345 Affects the Invasive Capacity of Pancreatic Ductal Adenocarcinoma Cell Lines by Suppressing MUC1 and TJP2 Expression

• Material Type: 博士論文
• Title: Overexpression of microRNA-345 Affects the Invasive Capacity of Pancreatic Ductal Adenocarcinoma Cell Lines by Suppressing MUC1 and TJP2 Expression
• Author/Editor: Tatsumi, Shigenobu; Fujii, Tomomi; Sugimoto, Aya; Sekita-Hatakeyama, Yoko; Morita, Kohei; Uchiyama, Tomoko; Itami, Hiroe; Takeda, Maiko; Yamazaki, Masaharu; Sho, Masayuki; Ohbayashi, Chiho
• Author Heading: Tatsumi, Shigenobu; Fujii, Tomomi; Sugimoto, Aya; Sekita-Hatakeyama, Yoko; Morita, Kohei; Uchiyama, Tomoko; Itami, Hiroe; Takeda, Maiko; Yamazaki, Masaharu; Sho, Masayuki; Ohbayashi, Chiho
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-05-25
• Publication Date (W3CDTF): 2022-05-25
• Alternative Title: microRNA-345の過剰発現は、MUC1およびTJP2の発現を抑制することにより、膵管腺癌細胞株の浸潤能に影響を及ぼす
• Periodical title: Applied Sciences
• No. or year of volume/issue: 12 11
• Volume: 12
• Issue: 11
• Pages: Article No.5351-
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2023-03-15
• Dissertation Number: 甲第866号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: PDAC; MUC1; TJP2; miR-345; cell proliferation; invasion
• Target Audience: 一般
• Note (General): type:Thesis; The majority of pancreatic carcinomas are pancreatic ductal adenocarcinomas (PDAC), and the presence of non-invasive pancreatic intraepithelial neoplasia or intraductal papillary mucinous neoplasm, as an associated lesion, is considered important. These microscopic hyperplastic or grossly papillomatous lesions exhibit varying degrees of morphological atypia and may develop into invasive carcinomas. In this study, we investigated whether mucin-1 (MUC1) is involved in the progression of pancreatic carcinoma and examined the mechanisms by which microRNAs regulate MUC1 expression in vitro. In PDAC cell lines, suppression of MUC1 expression reduced cell proliferation and invasion; PDAC cell lines transfected with an miR-345 precursor suppressed the expression of MUC1, and reduced cell proliferation and invasion. Tight junction protein 2 (TJP2), a putative target of miR-345, is regulated by MUC1. The suppression of TJP2 expression reduced cell proliferation by inducing apoptosis. These results suggest that MUC1 and TJP2, the putative target molecules of miR-345, are critical in maintaining the invasive potential of pancreatic carcinoma cells, and regulating their expression may prevent the progression of non-invasive pancreatic intraductal lesions to invasive carcinomas. This study provides new insights for the development of novel molecular targeted therapies for pancreatic carcinomas.; 博士（医学）・甲第866号・令和5年3月15日; © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).; identifier:Applied Sciences Vol.12 No.11 Article No.5351 (2022 May); identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4098; identifier:Applied Sciences, 12(11): Article No.5351
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4098/1/01%e7%94%b2866%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4098/2/02%e7%94%b2866%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4098/6/03%e7%94%b2866%e6%9c%ac%e6%96%87.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4098/7/04%e7%94%b2866Suppl.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3390/app12115351
• Related Material (NCID): BC10845134; https://ci.nii.ac.jp/ncid/BC10845134
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU