Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum

• Material Type: 博士論文
• Title: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum
• Author/Editor: Kawabe, Yoshie; Tanaka, Tatsuhide; Isonishi, Ayami; Nakahara, Kazuki; Tatsumi, Kouko; Wanaka, Akio
• Author Heading: Kawabe, Yoshie; Tanaka, Tatsuhide; Isonishi, Ayami; Nakahara, Kazuki; Tatsumi, Kouko; Wanaka, Akio
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2022-09
• Publication Date (W3CDTF): 2022-09
• Alternative Title: マウス脳梁グリア細胞の加齢や再ミエリン化に対する反応解析
• Periodical title: Neurochemical Research
• No. or year of volume/issue: 47 9
• Volume: 47
• Issue: 9
• Pages: 2826-2838
• ISSN (Periodical Title): ISSN : 0364-3190
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2024-03-14
• Dissertation Number: 甲第900号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Corpus callosum; Oligodendrocyte; Microglia; Astrocyte; Remyelination
• Target Audience: 一般
• Note (General): type:Thesis; Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells. In aged mice, premyelinating oligodendrocytes were decreased, but microglia continued to be present in the LAs. These results suggest that the presence of microglia is important for the characteristic cell localization pattern of LAs. In a cuprizone-induced demyelination model, we observed re-formation of LAs after completion of cuprizone treatment, concurrent with remyelination. These re-formed LAs again contained more microglia than the isolated cells. This finding supports the hypothesis that microglia contribute to the formation and maintenance of LAs. In addition, regardless of the distribution of cells (LAs or isolated cells), astrocytes were found to be more abundant than in the normal corpus callosum at 24 weeks after cuprizone treatment when remyelination is completed. This suggests that astrocytes are involved in maintaining the functions of remyelinated white matter.; 権利情報：© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11064-022-03676-z; identifier:Neurochemical Research. 2022 Sep, vol.47, no.9, p.2826-2838; identifier:0364-3190; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4351; identifier:Neurochemical Research, 47(9): 2826-2838
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/1/01%e7%94%b2900%e6%9c%ac%e6%96%87.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/2/02%e7%94%b2900%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/3/03%e7%94%b2900%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/8/04%e7%94%b2900Supplementary%20Figure1.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/9/05%e7%94%b2900Supplementary%20Figure2.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4351/10/06%e7%94%b2900Supplementary%20Table.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1007/s11064-022-03676-z
• Periodical Title (NCID): AA0075462X; https://ci.nii.ac.jp/ncid/AA0075462X
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU