Alteration in melanin content in retinal pigment epithelial cells upon hydroquinone exposure

• Material Type: 博士論文
• Title: Alteration in melanin content in retinal pigment epithelial cells upon hydroquinone exposure
• Author/Editor: Nishiyama, Takeyuki; Tsujinaka, Hiroki; Ueda, Tetsuo; Ogata, Nahoko
• Author Heading: Nishiyama, Takeyuki; Tsujinaka, Hiroki; Ueda, Tetsuo; Ogata, Nahoko
• Publication, Distribution, etc.: MDPI
• Publication Date: 2023-11
• Publication Date (W3CDTF): 2023-11
• Alternative Title: ハイドロキノン曝露による網膜色素上皮細胞のメラニン含量の変化
• Periodical title: International Journal of Molecular Sciences
• No. or year of volume/issue: 24 23
• Volume: 24
• Issue: 23
• Pages: 16801-
• ISSN (Periodical Title): ISSN : 1661-6596
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2024-03-14
• Dissertation Number: 甲第920号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: neovascular age-related macular degeneration; melanin production; hydroquinone; vascular endothelial growth factor; blue light
• Target Audience: 一般
• Note (General): type:Thesis; Abnormal pigmentation or depigmentation of the retinal pigment epithelium (RPE) is a precursor to neovascular age-related macular degeneration (nAMD). In this study, we evaluated the effects of hydroquinone (HQ), the most potent reductant in cigarette smoke, on the melanin production in RPE cells. Induced pluripotent stem cell (iPS)-derived RPE and adult retinal pigment epithelial (ARPE-19) cells were cultured with HQ. Real-time reverse transcription polymerase chain reaction revealed that the expression of melanin-related genes decreased due to the addition of HQ for 1 day. Enzyme-linked immunosorbent immunoassay showed that the concentration of melanin significantly decreased due to the addition of HQ for 24 h. A suspension of RPE cells with HQ for 24 h was prepared, and the absorbance was measured. The absorbance decreased particularly under blue light, suggesting that blue light may reach the choroid and cause choroidal inflammation. Additionally, melanin levels significantly decreased due to the addition of HQ for 1 week. After blue light irradiation on the RPE with HQ for 1 week, the vascular endothelial growth factor in the medium was significantly higher in the HQ group than in the control group. HQ-induced changes in melanin production may be responsible for the uneven pigmentation of the RPE, and these changes may cause nAMD.; 権利情報：© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).; identifier:International Journal of Molecular Sciences. 2023 Nov, vol.24, no.23, article no.16801; identifier:1661-6596; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4371; identifier:International Journal of Molecular Sciences, 24(23): 16801
• Source: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4371/1/01%e7%94%b2920%e6%9c%ac%e6%96%87.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4371/2/02%e7%94%b2920%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/4371/3/03%e7%94%b2920%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.3390/ijms242316801
• Periodical Title (NCID): AA12038549; https://ci.nii.ac.jp/ncid/AA12038549
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU