The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model

• Material Type: 博士論文
• Title: The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model
• Author/Editor: 松本, 亮
• Author Heading: 松本, 亮
• Publication, Distribution, etc.: SAGE Publications
• Publication Date: 2021-03-03
• Publication Date (W3CDTF): 2021-03-03
• Alternative Title: ブタ十二指腸内視鏡的粘膜下層剥離術モデルに対する自家筋芽細胞シートの穿孔抑制効果
• Periodical title: Cell Transplantation
• No. or year of volume/issue: 29
• Volume: 29
• Pages: 1-12
• ISSN (Periodical Title): ISSN : 0963-6897; ISSN : 1555-3892
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-03-03
• Dissertation Number: 甲医歯薬第1301号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: duodenum; neoplasm; endoscopic resection; regenerative medicine; cell sheet technology
• Target Audience: 一般
• Note (General): The recent advent of endoscopy has enabled the endoscopic submucosal dissection (ESD) of superficial nonampullary duodenal epithelial tumors. However, the substantially thin wall and presence of bile and pancreatic juice make it technically difficult to perform duodenal ESD without perforation, which leads to lethal complications. The present study evaluated the efficacy of autologous myoblast sheet transplantation for the prevention of late perforation after duodenal ESD in a porcine model. Two weeks before ESD, skeletal muscle was surgically excised from the femur of pigs, and myoblasts were isolated and seeded in temperature-responsive culture dishes to prepare sheets. Immediately after ESD, the autologous myoblast sheets were attached to the serosal surface at the ESD site with omentopexy. The pigs were divided into two groups: the autologous myoblast sheet group (n ¼ 5), where the myoblast cell sheet was attached to the ESD ulcer part from the duodenal serous side, and the Omentum group (n ¼ 5), where only the omentum was used. The pigs were sacrificed and analyzed macroscopically and histologically on postoperative day 3. The macroscopic examination of the abdominal cavity revealed perforation in the ESD ulcer area and leakage of bile in the Omentum group but no perforation in the Sheet group. A histopathological examination revealed that continuity of the duodenal wall at the ESD site was maintained with dense connective tissue in the Sheet group. In conclusion, autologous myoblast sheets were useful for preventing perforation after duodenal ESD.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1301号 学位授与年月日:令和3年3月3日; Author: Ryo Matsumoto, Kengo Kanetaka, Yasuhiro Maruya, Shun Yamaguchi, Shinichiro Kobayashi, Daisuke Miyamoto, Ken Ohnita, Yusuke Sakai, Keiichi Hashiguchi, Kazuhiko Nakao and Susumu Eguchi; Citation: Cell Transplantation, 29(1–12),pp.1-12 ; 2020; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-03-03)
• Source: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=26544&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
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• Related Material: http://hdl.handle.net/10069/40570
• Related Material (DOI): 10.1177/0963689720963882
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ