Gene-activated matrix harboring a miR20a-expressing plasmid promotes rat cranial bone augmentation

• Material Type: 博士論文
• Title: Gene-activated matrix harboring a miR20a-expressing plasmid promotes rat cranial bone augmentation
• Author/Editor: 四道, 玲奈
• Author Heading: 四道, 玲奈
• Publication, Distribution, etc.: Oxford University Press and Chinese Society for Biomaterials
• Publication Date: 2021-03-03
• Publication Date (W3CDTF): 2021-03-03
• Alternative Title: miR20a発現プラスミドを搭載した遺伝子活性化基質はラット頭蓋骨増生を促進する
• Periodical title: Regenerative Biomaterials
• No. or year of volume/issue: 8 2
• Volume: 8
• Issue: 2
• Pages: 1-9
• ISSN (Periodical Title): ISSN : 2056-3418; ISSN : 2056-3426
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-03-03
• Dissertation Number: 甲医歯薬第1313号
• Degree Type: 博士(歯学)
• Text Language Code: eng
• Subject Heading: gene-activated matrix; in vivo gene transfer; bone augmentation; atelocollagen; mir20a
• Target Audience: 一般
• Note (General): Gene-activated matrix (GAM) has a potential usefulness in bone engineering as an alternate strategy for the lasting release of osteogenic proteins but efficient methods to generate non-viral GAM remain to be established. In this study, we investigated whether an atelocollagen-based GAM containing naked-plasmid (p) DNAs encoding microRNA (miR) 20a, which may promote osteogenesis in vivo via multiple pathways associated with the osteogenic differentiation of mesenchymal stem/progenitor cells (MSCs), facilitates rat cranial bone augmentation. First, we confirmed the osteoblastic differentiation functions of generated pDNA encoding miR20a (pmiR20a) in vitro, and its transfection regulated the expression of several of target genes, such as Bambi1 and PPARγ, in rat bone marrow MSCs and induced the increased expression of BMP4. Then, when GAMs fabricated by mixing 100 μl of 2% bovine atelocollagen, 20 mg β-TCP granules and 0.5 mg (3.3 μg/μl) AcGFP plasmid-vectors encoding miR20a were transplanted to rat cranial bone surface, the promoted vertical bone augmentation was clearly recognized up to 8 weeks after transplantation, as were upregulation of VEGFs and BMP4 expressions at the early stages of transplantation. Thus, GAM-based miR delivery may provide an alternative non-viral approach by improving transgene efficacy via a small sequence that can regulate the multiple pathways.; 長崎大学学位論文 学位記番号:博（医歯薬）甲第1313号 学位授与年月日:令和3年3月3日; Author: Rena Shido, Yoshinori Sumita, Masahito Hara, Mayumi Iwatake, Shun Narahara, Mayumi Umebayashi, Kei-ichiro Miura, Yukinobu Kodama, Izumi Asahina; Citation: Regenerative Biomaterials, 8(2), pp.1-9; 2021; identifier:Nagasaki University (長崎大学), 博士(歯学) (2021-03-03)
• Source: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=26557&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): ©The Author(s) 2021. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• Related Material: http://hdl.handle.net/10069/00040582
• Related Material (DOI): 10.1093/rb/rbaa060
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ