Multifaceted array-based keloidal gene expression profiling reveals specific MDFI upregulation in keloid lesions

• Material Type: 博士論文
• Title: Multifaceted array-based keloidal gene expression profiling reveals specific MDFI upregulation in keloid lesions
• Author/Editor: 浅井, 幸
• Author Heading: 浅井, 幸
• Publication, Distribution, etc.: John Wiley & Sons, Ltd.
• Publication Date: 2021-06-02
• Publication Date (W3CDTF): 2021-06-02
• Alternative Title: マイクロアレイ解析に基づいたケロイド遺伝子発現プロファイリングにより、ケロイド病変部におけるMDFIの発現増加を明らかにした
• Periodical title: Clinical and Experimental Dermatology
• No. or year of volume/issue: 10.1111/ced.14698
• Volume: 10.1111/ced.14698
• ISSN (Periodical Title): ISSN : 0307-6938
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-06-02
• Dissertation Number: 甲医歯薬第1349号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: keloid; microarray analysis; MDFI; KALT
• Target Audience: 一般
• Note (General): Summary Background Keloid lesions are characterized by mesenchymal cell proliferation and excessive extracellular matrix deposition. Previous microarray analyses have been performed to investigate the mechanism of keloid development. However, the molecular pathology that contributes to keloid development remains obscure. Objective Exploring the underlying essential molecules of keloids using microarrays. Methods We performed microarray analyses of keloid and non-lesional skin tissues both in vivo and in vitro. Gene expression levels were compared between tissues and cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunopathological staining were used to determine the expression levels of molecules of interest in keloid tissues. Results Several common molecules were upregulated in both keloid tissues and keloid-lesional fibroblasts. PTPRD and NTM were upregulated both in vivo and in vitro. MDFI and ITGA4 were located at the center of the gene co-expression network analysis using keloid tissuess. qRT-PCR revealed significant expression levels of PTPRD and MDFI in keloid tissues. Immunopathological staining revealed that MDFI-positive cells, which have fibroblast characteristics, were located in the keloid-associated lymphoid tissue (KALT) portion of the keloid tissue. Conclusions Our gene expression profiles of keloids distinguished the difference between lesional tissue and cultured lesional fibroblasts, and MDFI was commonly expressed in both tissues and cells. Thus, MDFI-positive cells, which were located in the KALT, might be used in in vitro keloid studies and may play an important role in keloid pathogenesis.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1349号 学位授与年月日:令和3年6月2日; Author: M. Asai, Y. Koike, Y. Kuwatsuka, Y. Yagi, K. Kashiyamam, K. Tanaka, H. Mishima, K. Yoshiura, A. Utani, H. Murota; Citation: Clinical and Experimental Dermatology, Article in Press; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-06-02)
• Source: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=26669&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=26669&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): © 2021 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Clinical and Experimental Dermatology, Article in Press; 2021, which has been published in final form at https://doi.org/10.1111/ced.14698. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• Related Material: http://hdl.handle.net/10069/00040832
• Related Material (DOI): 10.1111/ced.14698
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ