A Combination of GM-CSF and Released Factors from Gamma-Irradiated Tumor Cells Enhances the Differentiation of Macrophages from Bone Marrow Cells and Their Antigen-Presenting Function and Polarization to Type 1

• Material Type: 博士論文
• Title: A Combination of GM-CSF and Released Factors from Gamma-Irradiated Tumor Cells Enhances the Differentiation of Macrophages from Bone Marrow Cells and Their Antigen-Presenting Function and Polarization to Type 1
• Author/Editor: Chen, Lichao
• Author Heading: Chen, Lichao
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-09-17
• Publication Date (W3CDTF): 2021-09-17
• Alternative Title: GM-CSFとガンマ線照射腫瘍細胞の放出因子の組み合わせによる骨髄細胞からのマクロファージ分化と、それらの抗原提示機能および1型への極性化の促進
• Periodical title: Medicines
• No. or year of volume/issue: 8 7
• Volume: 8
• Issue: 7
• Pages: 35-
• ISSN (Periodical Title): ISSN : 2305-6320
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-09-17
• Dissertation Number: 甲医歯薬第1359号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: GM-CSF; macrophage; radiotherapy
• Target Audience: 一般
• Note (General): Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes dendritic cell differentiation from precursors, and consequently, enhances the antigen presentation process and adaptive immune responses. With such functions, GM-CSF has been used as immunotherapy in combination with radiotherapy for cancer treatment to augment the survival and activity of immune cells. However, an immune-suppressive tumor microenvironment may cause anergy of T cells. It has also been reported that GM-CSF contributes to the development of myeloid-derived suppressor cells from the precursors. In this study, to analyze the combined effect of GM-CSF and released factors from cancer cells after gamma-ray irradiation on bone marrow cell differentiation and dynamics, we established an in vitro culture system using mouse bone marrow cells, GM-CSF, and conditioned medium from gamma ray irradiated mouse melanoma B16 cells at 24 Gy. We analyzed the gene expression changes of the bone marrow-derived cells on day 6. The results showed that GM-CSF dose-dependently enhanced the differentiation of macrophages from bone marrow cells, their antigenpresenting function and polarization to type I. The results implied the induced macrophages from the bone marrow may potentially contribute to tumor immune responses in a systemic manner when GM-CSF is boosted during photon-beam radiation therapy.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1359号 学位授与年月日:令和3年9月17日; Author: Lichao Chen, Shoji Imamichi, Ying Tong, Yuka Sasaki, Takae Onodera, Satoshi Nakamura, Hiroshi Igaki, Jun Itami, Mitsuko Masutani; Citation: Medicines, 8(7), art. no. 35; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-17)
• Source: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27084&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• Related Material: http://hdl.handle.net/10069/00040946
• Related Material (DOI): 10.3390/medicines8070035
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ