Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

• Material Type: 博士論文
• Title: Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells
• Author/Editor: 石井, 光寿
• Author Heading: 石井, 光寿
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-12-01
• Publication Date (W3CDTF): 2021-12-01
• Alternative Title: 脂肪由来間葉系幹細胞による肺上皮細胞間バリア機能増強効果
• Periodical title: Pharmaceutics
• No. or year of volume/issue: 13 8
• Volume: 13
• Issue: 8
• Pages: 1264-
• ISSN (Periodical Title): ISSN : 1999-4923
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-12-01
• Dissertation Number: 甲医歯薬第1376号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: mesenchymal stem/stromal cell; alveolar epithelial cell; barrier function; air-blood barrier; transepithelial electrical resistance; acute respiratory distress syndrome
• Target Audience: 一般
• Note (General): With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1376号 学位授与年月日:令和3年12月1日; Author: Mitsutoshi Ishii, Tomoshi Tsuchiya, Ryoichiro Doi, Yoichi Morofuji, Takashi Fujimoto, Hideki Muto, Takashi Suematsu, Ryoichi Mori, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige, Hironosuke Watanabe, Mayumi Iwatake and Takeshi Nagayasu; Citation: Pharmaceutics, 13(8), 1264; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01)
• Source: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27101&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27101&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• Related Material: http://hdl.handle.net/10069/00041137
• Related Material (DOI): 10.3390/pharmaceutics13081264
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ