Claudin-7 in keratinocytes is downregulated by the inhibition of HMG-CoA reductase and is highly expressed in the stratum granulosum of the psoriatic epidermis

• Material Type: 博士論文
• Title: Claudin-7 in keratinocytes is downregulated by the inhibition of HMG-CoA reductase and is highly expressed in the stratum granulosum of the psoriatic epidermis
• Author/Editor: 鍬塚, さやか
• Author Heading: 鍬塚, さやか
• Publication, Distribution, etc.: Elsevier B.V.
• Publication Date: 2022-03-02
• Publication Date (W3CDTF): 2022-03-02
• Alternative Title: ケラチノサイトのクローディン7は、乾癬表皮の顆粒層に高発現しており、HMG-CoA還元酵素阻害により発現が低下する
• Periodical title: Journal of Dermatological Science
• No. or year of volume/issue: 104 2
• Volume: 104
• Issue: 2
• Pages: 132-137
• ISSN (Periodical Title): ISSN : 0923-1811
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-03-02
• Dissertation Number: 甲医歯薬第1409号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: Claudin-7; Keratohyalin granule; Epidermal stratum granulosum; HMG-CoA reductase; Psoriasis
• Target Audience: 一般
• Note (General): Background: Cholesterol is de novo synthesized in the upper epidermis and plays an important role in maintaining the normality of skin. Studying the impact of the inhibition of cholesterol de novo synthesis in the epidermis may help understand how skin homeostasis is regulated.Objective: In this study, we created a gene expression profile to investigate the effect of hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors on epidermal homeostasis.Methods: A microarray analysis was performed using normal keratinocytes with or without HMG-CoA reductase inhibitor (pitavastatin) treatment. Real-time PCR confirmed the reproducibility of genes with altered expression in keratinocytes treated with HMG-CoA reductase inhibitors. Among these genes, we focused on reduced expression of claudin 7 histologically confirmed by immunohistochemical staining, in situ hybridization, and immunoelectron microscopy.Results: Claudin-7 was highly expressed in the stratum granulosum of psoriatic lesions but was not expressed in the normal epidermis. Immunoelectron microscopy revealed that claudin-7 was localized in the keratohyalin granules of psoriatic lesions.Conclusion: These results indicate that claudin-7 expression was regulated by HMG-CoA reductase in the epidermis and might play a pathogenic role in the keratohyalin granules found in the epidermal granular layer of psoriasis.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1409号 学位授与年月日:令和4年3月2日; Author: Sayaka Kuwatsuka, Yuta Koike, Yutaka Kuwatsuka, Toshifumi Yamaoka, Hiroyuki Murota; Citation: Journal of Dermatological Science, 104(2), pp. 132-137; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-03-02)
• Source: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27380&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27380&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): © 2021 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.
• Related Material: http://hdl.handle.net/10069/00041412
• Related Material (DOI): 10.002
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ