Donor and recipient adipose-derived mesenchymal stem cell therapy for rat lung transplantation

• Material Type: 博士論文
• Title: Donor and recipient adipose-derived mesenchymal stem cell therapy for rat lung transplantation
• Author/Editor: 下山, 孝一郎
• Author Heading: 下山, 孝一郎
• Publication, Distribution, etc.: Elsevier Inc.
• Publication Date: 2022-09-07
• Publication Date (W3CDTF): 2022-09-07
• Alternative Title: ドナーおよびレシピエント脂肪由来の間葉系幹細胞療法を用いたラット肺移植
• Periodical title: Transplantation Proceedings
• ISSN (Periodical Title): ISSN : 0041-1345
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-09-07
• Dissertation Number: 甲医歯薬第1464号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Background: Mesenchymal stem cells (MSCs) are beginning to be proven as immunosuppressant in the field of organ transplantation. However, the effects of MSC origin (donor or recipient) on immunosuppression are not clear. Hence, we investigated the effects of recipient and donor adipose-derived MSCs (ADMSCs) on immunosuppression in a rat lung transplantation model.Methods: Subjects were divided into no treatment, tacrolimus administration, recipient ADMSC administration, donor ADMSC administration, and mixed donor and recipient ADMSC administration groups. ADMSC-administered groups were also treated with tacrolimus. Histologic study, immunofluorescence, immunohistochemistry, enzyme-linked immunosorbent assay, and polymerase chain reaction were used for various analyses.Results: Fluorescently labeled ADMSCs were predominant in the grafted donor lung, but not in the recipient lung, on day 5. On day 7, the pathologic rejection grades of the grafted donor lung were significantly lower in the ADMSC-administered groups (P < .05) and did not differ among these groups. Although serum hepatocyte growth factor and vascular endothelial growth factor levels did not differ among the groups, interleukin 10 level was slightly higher in the ADMSC-administered groups. The numbers of infiltrating regulatory T cells in the grafted lung were significantly higher in the ADMSC-administered groups (P < .05) but did not differ with cell origin. Transcriptional analysis suggested interleukin 6 suppression to be the main overlapping immunosuppressive mechanism, regardless of origin. Therefore, a donor or recipient origin may not influence the immunosuppressive efficacy of ADMSCs in our rat lung transplantation model.Conclusions: Collectively, the results indicate that allogenic ADMSCs, regardless of their origin, may exert similar immunosuppressive effects in clinical organ transplantation.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1464号 学位授与年月日:令和4年9月7日; Author: Koichiro Shimoyama, Tomoshi Tsuchiya, Hironosuke Watanabe, Abdelmotagaly Ergalad, Mayumi Iwatake, Takuro Miyazaki, Yasumasa Hashimoto, Yu-I. Hsu, Go Hatachi, Keitaro Matsumoto, Mitsutoshi Ishii, Satoshi Mizoguchi, Ryoichiro Doi, Koichi Tomoshige, Tetsuji Yamaoka, and Takeshi Nagayasu; Citation: Transplantation Proceedings, article in press; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-09-07)
• Source: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27740&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27740&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): © 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
• Related Material: http://hdl.handle.net/10069/00041830
• Related Material (DOI): 10.1016/j.transproceed.2022.05.038
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ