Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence

• Material Type: 博士論文
• Title: Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence
• Author/Editor: 氏家, 里紗
• Author Heading: 氏家, 里紗
• Publication, Distribution, etc.: Radiation Research Society
• Publication Date: 2023-03-20
• Publication Date (W3CDTF): 2023-03-20
• Alternative Title: 抗CENP-C抗体による蛍光免疫二動原体染色体解析法：放射線量依存性、検証評価、姉妹セントロメア蛍光強度の放射線量応答性
• Periodical title: Radiation Research
• No. or year of volume/issue: 199 1
• Volume: 199
• Issue: 1
• Pages: 74-82
• ISSN (Periodical Title): ISSN : 0033-7587
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2023-03-20
• Dissertation Number: 甲医歯薬第1525号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Dicentric chromosome assay (DCA) is the most accepted cytological technique for the purpose of biological dosimetry in radiological and nuclear accidents, however, it is not always easy to evaluate dicentric chromosomes because of the technical difficulty in identifying dicentric chromosomes on Giemsa-stained metaphase chromosome samples. Here, we applied an antibody recognizing centromere protein (CENP) C, CENP-C, whose antigenicity is resistant to the fixation with Carnoy's solution. Normal human diploid cells were irradiated with various doses of 137Cs γ rays at 1 Gy/ min, treated with hypotonic solution, fixed with Carnoy's fixative, and metaphase chromosome spreads were stained with anti-CENP-C antibody. Dose-dependent induction of dicentric chromosomes was confirmed between 1 and 10 Gy of γ rays, and the results were compatible with those obtained by the conventional Giemsa-stained chromosome samples. The CENP-C assay also uncovered the difference in the fluorescence from the sister centromeres on the same chromosome, which was more pronounced after radiation exposure. Although the underlying mechanism is still to be determined, the result suggests a novel effect of radiation on centromeres. The innovative protocol for CENP-C-based DCA, which enables ideal visualization of centromeres, is simple, effective and reliable. It does not require skilled examiners, so that it may be an alternative method, avoiding uneasiness of the current DCA using Giemsa-stained metaphase chromosome samples.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1525号 学位授与年月日:令和5年3月20日; Author: Ujiie Risa, Kawamura Kasumi, Yamashita Shunichi, Mitsutake Norisato, Suzuki Keiji; Citation: Radiation Research, 199(1), pp.74-82; 2023; identifier:Nagasaki University (長崎大学), 博士(医学) (2023-03-20)
• Source: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=28231&item_no=1&attribute_id=70&file_no=1
• Access Restrictions: インターネット公開
• Rights (production): ⓒ 2023 by Radiation Research Society. All rights of reproduction in any form reserved.
• Related Material: http://hdl.handle.net/10069/00042266
• Related Material (DOI): 10.1667/RADE-22-00050.1
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 長崎大学 : 長崎大学学術研究成果リポジトリ