Translational Repression of a Splice Variant of Cynomolgus Macaque CXCL1L by Its C-terminal Sequence

• Material Type: 文書・図像類
• Title: Translational Repression of a Splice Variant of Cynomolgus Macaque CXCL1L by Its C-terminal Sequence
• Author/Editor: Nomiyama, Hisayuki; Osada, Naoki; Takahashi, Ichiro; Terao, Keiji; Yamagata, Kazuya; Yoshie, Osamu; 野見山, 尚之; 長田, 直樹; 高橋, 一郎; 寺尾, 恵治; 山縣, 和也; 義江, 修
• Author Heading: Nomiyama, Hisayuki; Osada, Naoki; Takahashi, Ichiro; Terao, Keiji; Yamagata, Kazuya; Yoshie, Osamu; 野見山, 尚之; 長田, 直樹; 高橋, 一郎; 寺尾, 恵治; 山縣, 和也; 義江, 修
• Publication, Distribution, etc.: Mary Ann Liebert
• Publication Date: 2017-03-17
• Publication Date (W3CDTF): 2017-03-17
• Periodical title: Journal of Interferon & Cytokine Research
• No. or year of volume/issue: 37 3
• Volume: 37
• Issue: 3
• Pages: 129-138
• ISSN (Periodical Title): ISSN : 1079-9907
• Text Language Code: eng
• Subject Heading: chemokine; CXCL1L; translational regulation; gene duplication; evolution; cynomolgus macaque; Old World monkeys; ケモカイン; 翻訳制御; 遺伝子重複; 進化; カニクイザル; 旧世界ザル
• Target Audience: 一般
• Note (General): type:プレプリント; We previously isolated a cDNA clone from cynomolgus macaque encoding a novel CXC chemokine that we termed CXCL1L from its close similarity to CXCL1. However, the cDNA consisted of three exons instead of four exons typically seen in other CXC chemokines. Here we isolated a cDNA encoding the full length variant of CXCL1L that we term CXCL1Lβ. CXCL1Lβ is 50 amino acids longer than the original CXCL1L, which we now term CXCL1Lα. The CXCL1Lβ mRNA is much more abundantly expressed in the cynomolgus macaque tissues than CXCL1Lα mRNA. However, CXCL1Lβ protein was poorly produced by transfected cells compared with that of CXCL1Lα. When the coding region of the 4th exon was fused to the C-terminus of CXCL1 or even to a non-secretory protein firefly luciferase, the fused proteins were also barely produced although the mRNAs were abundantly expressed. The polysome profiling analysis suggested that the inhibition was mainly at the translational level. Furthermore, we demonstrated the C-terminal five amino acids of CXCL1Lβ were critical for the translational repression. The present study thus reveals a unique translational regulation controlling the production of a splicing variant of CXCL1L. Since the CXCL1L gene is functional only in the Old World monkeys, we also discuss possible reasons for the conservation of the active CXCL1L gene in these monkeys during the primate evolution.; identifier:https://www.liebertpub.com/doi/10.1089/jir.2016.0085
• Format (IMT): application/pdf
• Source: https://kumadai.repo.nii.ac.jp/?action=repository_action_common_download&item_id=29684&item_no=1&attribute_id=21&file_no=1
• Access Restrictions: 限定公開
• Related Material (DOI): 10.1089/jir.2016.0085
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 熊本大学 : 熊本大学学術リポジトリ