細菌の鉄獲得機構阻害による静菌効果と臨床応用に向けた試み

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Author: 森崎, 浩ほか

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Publication date: 2013

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Note (General)：: type:text本研究では、neutrophil gelatinase-associated lipocalin (NGAL)の細菌鉄獲得阻害機能(静菌効果)に着目し、臨床的に問題となる感染症起因菌に対するNGALの静菌効果を検討するため臨床検体より高頻度に単離されるグラム陰性菌の同定調査を行い、...

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科研費研究者番号 : 60182226

科研費研究者番号 : 20348767

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Material Type: 文書・図像類
Title: 細菌の鉄獲得機構阻害による静菌効果と臨床応用に向けた試み
Author/Editor: 森崎, 浩
山田, 高成
Author Heading: 森崎, 浩
山田, 高成
Publication Date: 2013
Publication Date (W3CDTF): 2013
Alternative Title: サイキンノテツカクトクキコウソガイニヨルセイキンコウカトリンショウオウヨウニムケタココロミ
Saikin no tetsu kakutoku kiko sogai ni yoru seikin koka to rinsho oyo ni muketa kokoromi
Bacteriostatic effects of neutrophil gelatinase-associated lipocalin against clinically identified bacteria
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
Subject Heading: 感染症,NGAL,静菌効果
Target Audience: 一般
Note (General): type:text
本研究では、neutrophil gelatinase-associated lipocalin (NGAL)の細菌鉄獲得阻害機能(静菌効果)に着目し、臨床的に問題となる感染症起因菌に対するNGALの静菌効果を検討するため臨床検体より高頻度に単離されるグラム陰性菌の同定調査を行い、大腸菌(E.coli)、肺炎桿菌、エンテロバクター・クロアカエの上位3菌種を同定した。細菌のTuf遺伝子を標的としたreal-time PCR法では、NGAL15μgの添加でE.coliの3時間後の増殖が抑制される傾向を認め、高濃度NGALがE. coliに対して増殖能を抑制する可能性が示唆された。 We previously focused on bacteriostatic effects of neutrophil gelatinase-associated lipocalin (NGAL), known as a biomarker of acute kidney injury, and investigated the interaction between this protein and intestinal microflora. The present study demonstrated that bacteriostatic effects of NGAL against bacteria, which is frequently found in clinical situations. As a result, gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae was investigated to examine the bacteriostatic effects of NGAL in vitro. To detect bacterial growth, real-time PCR by using bacteria specific primer was performed. Although 1 mcg NGAL did not prevent the growth of these bacteria in vitro, 15 mcg NGAL tended to inhibit the growth of Escherichia coli, but it did not reach statistically significance. In conclusion, the present study demonstrated that high concentration NGAL might be able to inhibit the growth of Escherichia coli.
研究種目 : 挑戦的萌芽研究研究期間 : 2012～2013 課題番号 : 24659801 研究分野 : 医歯薬学科研費の分科・細目 : 外科系臨床医学・救急医学
Access Restrictions: 限定公開
Related Material: 科研費研究者番号 : 60182226
科研費研究者番号 : 20348767
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ