赤血球が刻む概日リズム測定系の開発

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Author: 大澤, 要介

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Publication date: 2013

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Note (General)：: type:text本研究では、生物発光共鳴エネルギー移動(Bioluminescence resonance energy transfer: BRET)を用いて、ペルオキシレドキシン2(PRX2)二量体の形成を経時的にモニタリングできる測定系を開発した。本研究からPRX2の酸化還元リズムは過酸化...

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科研費研究者番号 : 50528429

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Material Type: 文書・図像類
Title: 赤血球が刻む概日リズム測定系の開発
Author/Editor: 大澤, 要介
Author Heading: 大澤, 要介
Publication Date: 2013
Publication Date (W3CDTF): 2013
Alternative Title: セッケッキュウガキザムガイジツリズムソクテイケイノカイハツ
Sekkekkyu ga kizamu gaijitsu rizumu sokuteikei no kaihatsu
Development of a novel system measuring a circadian rhythm of erythrocytes
Periodical title: 科学研究費補助金研究成果報告書
Text Language Code: jpn
Subject Heading: ペルオキシレドキンシン,概日リズム,赤血球,BRET
Target Audience: 一般
Note (General): type:text
本研究では、生物発光共鳴エネルギー移動(Bioluminescence resonance energy transfer: BRET)を用いて、ペルオキシレドキシン2(PRX2)二量体の形成を経時的にモニタリングできる測定系を開発した。本研究からPRX2の酸化還元リズムは過酸化水素が同調因子となっていることが示唆された。核も時計遺伝子も持たない赤血球のPRX2の酸化還元リズムの制御機構の解明は、液性・神経性の概日シグナルによる末梢時計の制御システムや、末梢組織間の多様な内的脱同調のあり方の理解に大きく寄与する。 I developed a measuring system based on bioluminescence resonance energy transfer (BRET). The system can sequentially monitor the homodimer formation of Peroxyredoxin 2 (PRX2). The temporal resolution of the luminescent data was dramatically improved by measuring the PRX2 dimeric rhythm at 10-minute intervals, which enabled us to calculate the acrophase or free-running period from the luminescent data with high accuracy. This research suggested that hydrogen peroxide acts as an entraining agent (Zeitgeber) on the oxidation-reduction rhythm of PRX2. The elucidation of the time-keeping mechanism of the redox PRX2 rhythm in erythrocytes which lack a cell nucleus and clock genes may contribute greatly to our understanding of how the humoral or neuronal circadian signals control the peripheral clocks, and how the internal desynchronization between peripheral tissues occurs.
研究種目 : 若手研究(B) 研究期間 : 2012～2013 課題番号 : 24791255 研究分野 : 医歯薬学科研費の分科・細目 : 内科系臨床医学・精神神経科学
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Related Material: 科研費研究者番号 : 50528429
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Original Data Provider (Database): 慶應義塾大学 : 慶應義塾大学学術情報リポジトリ